– ´´My´´ Monograph: Induction of benzonidazole resistance in human isolates of Trypanosoma cruzi @ Inflammatory processes may play role in ALS – FDA-approved drug shows promise against the neurodegenerative disease in mice @ Stem Cell Therapy Helps Broken Hearts Heal in Unexpected Way – Study in Nature Sheds New Light on Heart Attack Treatment Controversy @ Virgina Niemi – Not just a gut feeling: Improving animal models of colorectal cancer @ TBT 10/8/19 – The Mouse Cancer from vaping – news roundup – Jerome Adams MD @ Anti-tumor immune therapy for colon cancer works in mice, says new study & Altogen Labs Hs746T Xenograft Service Gastric Cancer @ https://en.wikipedia.org/wiki/Monograph @ ´´A strain, in reference to rodents, is a group in which all members are as nearly as possible genetically identical. In laboratory mice, this is accomplished through inbreeding. By having this type of population, it is possible to conduct experiments on the roles of genes, or conduct experiments that exclude genetic variation as a factor.´´ https://en.wikipedia.org/wiki/Laboratory_mouse @ VERY IMPORTANT INFORMATION LIKE VIDEOS, SOCIAL NETWORKS, WEBSITES, LINKS AND IMAGES

Nanomedicina –> Texto que redigi. Nanomedicine –> Text I typed

LISTA DE NOMES – PEOPLE´S NAMES – E-MAIL LIST – LISTA DE E-MAILS-converted – People´s names who liked of the e-mail list I did in 2015 and sent a positive feedback to me by e-mail. Nome das pessoas que gostaram da lista de e-mails que fiz em 2015 e me enviaram um feedback positivo pra mim pelo e-mail.

List of people who gave me a positive feedback by e-mail about an excellent, detailed and very extensive e-mail list I did in 2015 from 20 Best Universities of the World. This e-mail list is more related to the Area of Biological Sciences. I sent it to a very big amount of excellent professors, scientists and/or researchers of Brazil and other countries of the world. A summary about this Project (e-mail list) was sent to them too. Very important information about the e-mail list I did in 2015: A little more than 32,000 e-mails of researchers, professors and/or scientists were cataloged by me. It was a very hard work, of course. Very Important Note: I did not earn any money with this project nor with this blog. The sharing of this blog is fundamental to the World Scientific Community and other people worldwide, of course. I sent this e-mail list to many people of the Scientific Community in Brazil and other countries. After a while, I send an other e-mail to so much people like American and Brazilian Govern, informing that I didn’t ask permission from each of them to put their e-mail on the e-mail lists I did. Note: To do it, of course, it would be necessary to send an e-mail with the objectives of the e-mail list to each person that I wanted to put the e-mail address in the e-mail list. It would be an extremely hard work, of course, and the e-mail list would be done so much later with the different number of people in the e-mail list because as many people know, each one has your own opinion and thoughts about a subject, for example. And the other hand, I did the e-mail lists in 2022 related to people who are linked to Excellent Research and Teaching Centers of the World without asking permission to each person to I know if I can put the e-mail address in the email lists or not. So, I send e-mail messages to them informing this blog that has a very big amount of relevant information and knowledge like websites, links, YouTube Videos, images, social networks and other types of technologies and and informing a very important note that I don´t earn any money from this blog. I also informed in the e-mail message to them that if she or he no longer wanted to receive my e-mails, he or she just reply me Unsubscribe. Comparing with the total number of e-mails cataloged by me in these new e-mail lists that I made in 2022 (I don’t know the number of e-mail addresses), a few people responded to me Unsubscribe by e-mail. 

It is very important the people worldwide live better and longer, more and more, by very efficient scientific researches and projects, resulting in new ideas to humanity like new platforms and websites to professors, students, researchers and/or scientists and/or other people worldwide, depending of the type and objectives of the inventions. 

It´s fundamental the invention of new vaccines, drugs, therapeutic substances, medical devices and other types of prevention, diagnostic, prognostic and treatment methods. Unfortunately there are so much diseases and there are the rare diseases, unknown diseases and fatal diseases. The Scientific Discoveries is extremely important to the world people, for sure. 

The Interaction between different Departments is very important, including the same types of Departments but from different Research Centers in different parts of the world in different aspects. However, projects on this subject will always be fundamental for national and global progress, for sure.

My intentions to do this e-mail list in 2015 and for sending it to many professors, scientists and/or researchers worldwide were and are always to contribute significantly to world progress, helping their work in anyway, in different situations, for example, to share ideas, research evidence, experiences and skills, not falling under data protection laws and fines, of course. Therefore, hitting the same key, coming back on the same subject, as many people know, the interactions among Academic Departments in different countries are very important to the world progress. So, these interactions among people need to be very efficient and faster, of course. It is very important the creation of very efficient platforms and websites to people who are linked to the Scientific Community, for sure. 

I NEVER want to harm myself either financially and/or psychologically with this e-mail list I made in 2015. For example, a professor may not or would not like an e-mail sent to him by a person with whom I sent the e-mail list that I did in 2015 even not falling under Laws that are linked to the protection of data and fine.

A interação entre diferentes Departamentos de Centros de Pesquisa é muito importante, inclusive os mesmos tipos de Departamentos só que de diferentes Centros de Pesquisa nas mais diversas partes do mundo nos seus diferentes aspectos. Contudo, projetos sobre este assunto sempre serão fundamentais para o progresso nacional e mundial. 

Do the downloads !!! Share!! Thanks!!

´´The world people need to have very efficient researches and projects resulting in very innovative drugs, vaccines, therapeutical substances, medical devices and other technologies according to the age, the genetics and medical records of the person. So, the treatment, disgnosis and prognosis will be very efficient and better, of course´´. Rodrigo Nunes Cal

https://atomic-temporary-144720889.wpcomstaging.com/2021/08/14/do-the-downloads-of-very-important-detailed-and-innovative-data-of-the-world-about-my-dissertation-like-the-graphics-i-did-about-the-variations-of-weights-of-all-mice-control/

Mestrado – Dissertation – Tabelas, Figuras e Gráficos – Tables, Figures and Graphics


Impact_Fator-wise_Top100Science_Journals

GRUPO_AF1

GRUPO_AF2

GRUPO AFAN 1

GRUPO AFAN 2

Slides – mestrado

CARCINÓGENO DMBA EM MODELOS EXPERIMENTAIS

Avaliação da influência da atividade física aeróbia e anaeróbia na progressão do câncer de pulmão experimental – Summary – Resumo

https://www.cincinnatichildrens.org/news/release/2019/stem-cell-therapy https://news.harvard.edu/gazette/story/2019/11/fda-approved-drug-shows-promise-against-als-in-mice/

https://atomic-temporary-144720889.wpcomstaging.com/2018/07/15/my-monography-chagas-disease-research-in-laboratory-2/

INDUÇÃO DE RESISTÊNCIA AO BENZONIDAZOL EM ISOLADOS HUMANOS DE TRYPANOSOMA CRUZI -> https://science1984.wordpress.com/wp-content/uploads/2018/03/monografia-monography.pdf

http://slideplayer.com.br/slide/7306497/

Gratidão – Gratitude – Vídeo – Video: Convites p/ eu participar de eventos científicos muito importantes do mundo em pouco tempo – Gratitude: I am very grateful because I was invited by Internet through direct messages to participate in 55 very important science events in the world in 25 cities in less than 1 year.

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Inflammatory processes may play role in ALS

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Health & Medicine

Inflammatory processes may play role in ALS

3d illustration of nervous network and nerve cells in blue.
Following treatment with an anti-inflammatory drug, the onset of ALS was delayed in a mouse model. ALS affects nerve cells (pictured) in the brain and spinal cord. iStock

FDA-approved drug shows promise against the neurodegenerative disease in mice

By MGH News and Public Affairs

DateNovember 27, 2019Share

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The cause of amyotrophic lateral sclerosis (ALS) — a disabling neurodegenerative disease that affects nerve cells in the brain and spinal cord and leads to weakened muscles and early death — is not fully understood, but accumulating evidence suggests that inflammatory processes may play a role in the initiation and progression of the condition.

In research led by investigators at Harvard-affiliated Massachusetts General Hospital (MGH) and published in Scientific Reports, treatment with an anti-inflammatory drug delayed the onset of disease in a mouse model of ALS.

Only two treatments are approved by the U.S. Food and Drug Administration (FDA) to treat ALS, and they are only modestly effective. Recent studies from MGH demonstrated that cromolyn sodium, an FDA-approved compound used to treat asthma and other conditions, exerts neuroprotective effects in cellular and animal models of Alzheimer’s disease.

To test the therapeutic potential of the drug against ALS, a team injected cromolyn sodium into male and female mice with and without a genetic mutation that causes ALS. The team was led by Ghazaleh Sadri-Vakili, director of the NeuroEpigenetics Laboratory at the Sean M. Healey and AMG Center for ALS and the MassGeneral Institute for Neurodegenerative Disease, in collaboration with Healey Center and MGH colleagues.

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In addition to observing that the treatment delayed the development of disease symptoms in the mice carrying the ALS mutation, the researchers demonstrated that it protected neurons from degenerating and helped maintain the connections between nerves and muscles. Also, cromolyn sodium reduced inflammation surrounding muscles by targeting specific immune cells, called mast cells, and decreased pro-inflammatory markers both in the spinal cord and the blood.

“Our study supports the notion that inflammation has a significant role in the progression of ALS and therefore exploring anti-inflammatory treatments may be of great value for developing an effective treatment,” said Sadri-Vakili. “Our findings demonstrate that cromolyn treatment provides neuroprotection in a mouse model of ALS. It remains to be seen whether these effects will translate to people living with the disease. Therefore, we will continue to explore inflammation’s role in disease development and progression in hopes of translating this research into potential ALS treatments.”

This work was supported by Project ALS.

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Stem Cell Therapy Helps Broken Hearts Heal in Unexpected Way

Study in Nature Sheds New Light on Heart Attack Treatment Controversy

Wednesday, November 27, 2019

Macrophages to the Rescue

A microscopic histology image of macrophage immune cells.

In this microscopic histology image, macrophage immune cells (shown in red and green) flock to the injured region of a damaged mouse heart three days after researchers injected adult heart stem cells within the yellow dotted area. Researchers report Nov. 27 in Nature that stem cell therapy helps hearts recover from heart attack by triggering an innate immune response that alters cell activity around the injured area so that it heals with a more optimized scar and improved contractile properties.

Stem cell therapy helps hearts recover from a heart attack, although not for the biological reasons originally proposed two decades ago that today are the basis of ongoing clinical trials. This is the conclusion of a Nov. 27 study in Nature that shows an entirely different way that heart stem cells help the injured heart – not by replacing damaged or dead heart cells as initially proposed.

The study reports that injecting living or even dead heart stem cells into the injured hearts of mice triggers an acute inflammatory process, which in turn generates a wound healing-like response to enhance the mechanical properties of the injured area.

Mediated by macrophage cells of the immune system, the secondary healing process provided a modest benefit to heart function after heart attack, according to Jeffery Molkentin, PhD, principal investigator, director of Molecular Cardiovascular Biology at Cincinnati Children’s Hospital Medical Center and a professor of the Howard Hughes Medical Institute (HHMI).

“The innate immune response acutely altered cellular activity around the injured area of the heart so that it healed with a more optimized scar and improved contractile properties,” Molkentin said. “The implications of our study are very straight forward and present important new evidence about an unsettled debate in the field of cardiovascular medicine.”

The new paper builds on a 2014 study published study by the same research team, also in Nature. As in that earlier study, the current paper shows that injecting c-kit positive heart stem cells into damaged hearts as a strategy to regenerate cardiomyocytes doesn’t work. The findings prompted Molkentin and his colleagues to conclude that there is a need to “re-evaluate the current planned cell therapy based clinical trials to ask how this therapy might really work.”

An Unexpected Discovery

The study worked with two types of heart stem cells currently used in the clinical trials—bone marrow mononuclear cells and cardiac progenitor cells. As the researchers went through the process of testing and re-verifying their data under different conditions, they were surprised to discover that in addition to the two types of stem cells, injecting dead cells or even an inert chemical called zymosan also provided benefit to the heart by optimizing the healing process. Zymosan is a substance designed to induce an innate immune response.

Researchers reported that stem cells or zymosan therapies tested in this study altered immune cell responses that significantly decreased the formation of extra cellular matrix connective tissue in the injury areas, while also improving the mechanical properties of the scar itself. The authors concluded: “injected hearts produced a significantly greater change in passive force over increasing stretch, a profile that was more like uninjured hearts.”

Molkentin and his colleagues also found that stem cells and other therapeutic substances like zymosan have to be injected directly into the hearts surrounding the area of infarction injury. This is in contrast to most past human clinical trials that for patient safety reasons simply injected stem cells into the circulatory system.

“Most of the current trials were also incorrectly designed because they infuse cells into the vasculature,” Molkentin explained. “Our results show that the injected material has to go directly into the heart tissue flanking the infarct region. This is where the healing is occurring and where the macrophages can work their magic.”

The researchers also noted an interesting finding involving zymosan, a chemical compound that binds with select pattern recognition receptors to cause an acute innate immune response. Using zymosan to treat injured hearts in mice resulted in a slightly greater and longer-lasting benefit on injured tissues than injecting stem cells or dead cell debris.

Looking to the Future

Molkentin said he and other collaborating scientists will follow up the findings by looking for ways to leverage the healing properties of the stem cells and compounds they tested.

For example, considering how heart stem cells, cell debris and zymosan all triggered an acute innate immune response involving macrophages in the current paper, Molkentin explained they will test a theory that harnesses the selective healing properties of macrophages. This includes polarizing or biologically queuing macrophages to only have healing-like properties.

Further testing of this, he said, could therapeutically be very important for developing future treatment strategies.

The study’s first author was Ronald Vagnozzi, PhD, a fellow and investigator in the Molkentin Lab. Key collaboration also came from scientists in the Cincinnati Children’s Heart Institute, the University of Cincinnati Department of Pediatrics and the Center for Systems Biology (Department of Imaging) and the Cardiovascular Research Center at Massachusetts General Hospital and Harvard Medical School in Boston.

Funding support for the study came in part by grants from the National Institutes of Health (R01 HL132391) and an NIH Research Service Award via the National Heart Blood and Lung Institute (F32 HL128083), the Howard Hughes Medical Institute, and a Career Development Award from the American Heart Association (19CDA34670044). Flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Core in the Division of Rheumatology at Cincinnati Children’s.

Contact Information

Nick Miller
513-803-6035
nicholas.miller@cchmc.org

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33 Comments

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