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Volume 8 • Issue 2 • 1000160 J Nanomedine Biotherapeutic Discov, an open access journal ISSN: 2155-983X Opinion Article Open Access Journal of Nanomedicine & nr uoJ Biotherapeutic Discovery al of Nanomedicine & Biotherapeutic D si cove yr ISSN: 2155-983X Cal, J Nanomedine Biotherapeutic Discov 2018, 8:2 DOI: 10.4172/2155-983X.1000160 Opinion

Nanomedicine is undoubtedly very promising because it involves high quality technologies and precision in the detection and treatment of diseases. Not so long ago this new branch of study and research emerged, that is, in general, it is recent. The increase in the financial investment in nanomedicine in the most diverse countries of the world is very important and essential in several aspects, as in the improvement of the quality of the scientific research carried out and in the laboratories involved, the greater hiring of qualified teachers and researchers, related in this new branch of medicine and among others. In this way, the chances of curing fatal diseases will increase and the types of treatment and diagnosis of the diseases will be better. Currently there are not a large number of nanomedicine teaching and research centers in the world, for this reason, the construction of highly modern and appropriate new places, the hiring of researchers, very well qualified professors and others involved and the improvement in all the aspects of existing teaching and research centres of nanomedicine are extremely important for the socio-economic development of the country. There are not many research and study centres that apply nanomedicine, so it is essential that there is a greater number of highly appropriate places of study and research of nanomedicine, with very skilled people and excellent technologies to apply the fundamentals, methodologies and objectives of nanomedicine in practice, making the results of the work very satisfactory. The manufacture of highly modern medical devices that indicate the result of the examination done quickly with high precision in the diagnosis is of fundamental importance for the life of the patient, emphasizing that the early diagnosis is essential for the success of the treatment of the disease. In addition, schools, colleges and universities need to better understand the applications, fundamentals and objectives of nanomedicine so that the knowledge generated from large and important scientific studies can be disseminated through various means of communication such as the internet, television, radio and other ways, and there are important partnerships between research and study centres in different countries, for example. That would make nanomedicine gain much more prominence in the world media by making more people show interest in working or investing financially, for example, sponsor, in this very promising industry. It is also very important that there is the dissemination of information related to nanomedicine in the centers of study and research in the world that did not divulge the importance of nanomedicine in the life of the human being. For this reason, politicians play a very important role in this regard, that is, there must be a greater awareness of the importance that nanomedicine brings to the patient, for example, in the elaboration of laws and projects that help the work of those who are involved in nanomedicine in the world and in the construction of research and study centers with many very modern technologies. Nanomedicine means the use of nanotechnology in methodologies, for example, the application of nanoprobes equipped with intelligent chip in the human body to detect in a more precise and fast way the disease and then treat it quickly as well. It is very important that there be methods such as vaccination to totally prevent the transmission of a certain disease to another person or from an animal to a person. Many high-quality research and testing centres have long used experimental animals to study pathologies in order to better understand the disease to discover new drugs, vaccines or other forms of disease prevention, detection and therapeutic resource. Unfortunately there are fatal diseases, even though there are many high quality scientific investigation. Therefore, the role that nanomedicine brings to the whole world society is undoubtedly of utmost importance for the benefit of the human being.

Nanomedicine: An Evolving Research Cal RN* Department of Biomedicine, Federal University of Triangulo Mineiro (Uberaba), Brazil *Corresponding author: Rodrigo Nunes Cal, Department of Biomedicine, Federal University of Triangulo Mineiro (Uberaba), Brazil, Tel: +55 34 3700-6000; E-mail: rodrigonunescal@yahoo.com Received July 12, 2018; Accepted July 27, 2018; Published August 07, 2018 Citation: Cal RN (2018) Nanomedicine: An Evolving Research. J Nanomedine Biotherapeutic Discov 8: 160. doi: 10.4172/2155-983X.1000160 Copyright: © 2018 Cal RN. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Am J Cardiovasc Dis. 2016; 6(3): 70–80.Published online 2016 Sep 15.PMCID: PMC5030387PMID: 27679742

Small mammalian animal models of heart disease

Paula Camacho,1Huimin Fan,2Zhongmin Liu,2 and Jia-Qiang He1Author informationArticle notesCopyright and License informationDisclaimerThis article has been cited by other articles in PMC.Go to:

Abstract

There is an urgent clinical need to develop new therapeutic approaches for treating cardiovascular disease, but the biology of cardiovascular regeneration is complex. Model systems are required to advance our understanding of the pathogenesis, progression, and mechanisms underlying cardiovascular disease as well as to test therapeutic approaches to regenerate tissue and restore cardiac function following injury. An ideal model system should be inexpensive, easily manipulated, reproducible, physiologically representative of human disease, and ethically sound. The choice of animal model needs to be considered carefully since it affects experimental outcomes and whether findings of the study can be reasonably translated to humans. This review presents a guideline for the commonly used small animal models (mice, rats, rabbits, and cats) used in cardiac research as an effort to standardize the most relevant procedures and obtain translatable and reproducible results.Keywords: Mouse, rat, rabbit, cat, myocardial infarction, heart failure, diabetic cardiomyopathyGo to:

Introduction

Cardiovascular disease is considered the major cause of morbidity and mortality worldwide. Over the past several years, enormous achievements have been made in the management of cardiovascular disease, which has depended on the use of experimental animal models [1]. However, there are still no permanent cures for most cardiovascular diseases. For example at present, the only cure for heart failure (HF) is a heart transplant; however, its therapeutic potential is limited by very small numbers of donor hearts available relative to the need and is complicated by long-term allograft vasculopathy. Tissue-engineered biomaterials and autologous or allogeneic cell transplant therapy may have the potential to restore function and obviate the necessity for transplant [1,2]). In recent years, gene therapy and cell transplant technologies have advanced remarkably. If proven efficient and safe, these therapies might revolutionize the treatment of myocardial failure [3].

A major challenge of preclinical testing is to establish clinically relevant models for myocardial failure and infarction [3]. Donated human hearts, either non-transplantable (ranging from healthy to diseased) or end-stage failing hearts, typically obtained at the time of transplantation, are great tools for addressing this issue. These tissues are, however, available in very limited quantities and exhibit great variability due to differences in factors such as genetics, medication, diet, social habits, and disease. Therefore, it is necessary to have a suitable animal model where cardiac physiology and disease can be studied efficiently and reliably with translational applicability to humans [4].

Depending on the cardiovascular process being studied, the choice of animal model needs to be considered carefully since it affects experimental outcomes and whether findings of the study can be reasonably translated to humans. As a simple rule, the closer the heart or body weight of the animal model to human heart or body weight, the more similar are the hearts [4]. Both small and large animal models of heart disease have advantages and disadvantages. The main problems with using small animals are how to establish functional benefit of the treatment and the translation of results. In large-animal models, the ethical requirement for limiting the number of animals influences study protocols, especially concerning non-human primates or dogs [3].

Determining the best experimental model of a human condition requires a number of decisions and compromises – especially in relation to obtaining the optimal balance between the quantity and quality of the data produced and the relevance of the data to the condition under investigation. In assessing the utility of an investigative model, it is necessary to identify the research objectives. The nature of the question under study will greatly influence selection of the most appropriate investigative models and endpoints of cardiac function and malfunction [5]. It is also important to standardize the procedures used so as to obtain relevant and reproducible results that can be compared with other findings.

Rodent models are often used in cardiovascular research since they are easier to handle and house, have a short gestation time, genetically manipulate to generate transgenic strains, and have low maintenance costs; therefore, they are more suitable for “high-throughput” studies than large animal models [6]. These characteristics make small rodent models the most used model for cardiac physiology and disease, genetics, pharmacology, and long-term survival studies [4]. However, rodents are phylogenetically very distant from humans and some pathophysiological features of disease and their response to pharmacological treatments may not be reliable predictors [4,6].Go to:

Mouse

During the past 15 years, the mouse has become the model organism of choice to study human heart disease. 99% of human genes have direct murine orthologs and mice are suitable for selection of genetically modified individuals within a relatively short time because of their high breeding rate. They also have a short life span, allowing investigators to follow the natural history of the disease at an accelerated pace [6]. Use of genetically modified mouse models allows for rapid establishment of proof-of-principle, which can later be extended into larger animal models and, eventually, into humans [4].

Despite their widespread use, mice represent a heart model that is farthest from human contractile function, mainly due to their small size and short lifespan. Thus, translational aspects and the value of genetic mouse models must be interpreted with caution. Although these models may recapitulate some of the characteristics of the human cardiac phenotype of a disease, they typically do not recapitulate all aspects of human cardiovascular disease [4].

Ligation-induced myocardial infarction (MI)

MI animal model is one of the most commonly used one to mimic human heart attack. Mortality after MI is relatively high and surviving patients are often severely compromised due to insufficient heart-pump function. At the cellular level, damage to the heart’s contractile constituents, the cardiomyocytes, is irreversible, and treatments merely serve to reduce symptoms. Only a minority of patients receives heart transplants [7].

The neonatal mouse MI model represents a useful tool for evaluating mammalian cardiac regeneration and cellular/molecular mechanisms that govern cardiomyocyte proliferative capacity only in the early stage of neonatal mice [8]. To generate MI, neonates are briefly sedated using an isoflurane induction chamber followed by a short cooling period (a few minutes) in ice water. The animals are then taped to a cooling bag in the right lateral position to ensure continued hypothermic anesthesia during the surgical procedure. The skin is cut a few millimeters below the left foreleg, the thorax opened in the 4th intercostal space, and the left anterior descending (LAD) artery is ligated. MI is indicated by a light pallor of the myocardium below the ligature after suturing. The musculoskeletal thorax and the skin are closed, and mice are put onto a 37°C heated pad to recover from the anesthesia [8–10].

It is commonly found that the complete regeneration of cardiomyocytes can be reached when the LAD artery is ligated in newborn mice. Myocardial necrosis is usually observed at Day 3 after ligation as ~75% of the myocardium below the ligature becomes nonviable. There is also a marked decline in left ventricular (LV) systolic function at Day 4 after injury. Cardiac systolic function is able to restore to sham operating levels at Day 21 after MI as the myocardium is repaired to greater than 95% viable [8]. However, the complete regeneration is age-dependent. For 1 day old mice, they are able to clear fibrotic tissue and replenish all cardiomyocytes while for 7 days old mice, their capacity for the capacity for complete morphological and functional cardiac regeneration was lost and retained significant scar tissue at day 21 after MI [9]. Very few proliferating cardiomyocytes were identified in 7 day old mice after MI and the remodeling response of 14 day old mice was characterized by extensive fibrosis, wall thinning, and ventricular dilation after MI [8].

The adult mouse infarct model better represents the cardiac response of human patients to an ischemic injury than neonatal mouse model. To induce heart infarction, mice are anesthetized by inhaling isoflurane or injecting anesthetics (e.g., Ketamine/Xylazine). The mice are then intubated using a catheter and ventilated with a rodent ventilator. The surgical procedure is similar to the one described above for neonatal mice. A left-sided thoracotomy is performed and the LAD artery is permanently ligated, approximately ~4 mm distal to the origin of the artery under the left atrium. Infarction is verified by LV blanching distal to the suture. The chest and skin are then closed, and mice are allowed to recover in a heated chamber [8,11,12]. Inflammation and scar tissue formation replacing the necrotic myocardium after MI are essential to preventing cardiac wall rupture. It is possible that ischemic injury, with subsequent cell necrosis, apoptosis, and damage to the extracellular matrix, dictates the course of inflammation and inhibits complete regeneration [13].

An inherent limitation of the LAD ligation model both in neonates and adults makes standardizing injury size difficult. Given that a proximal LAD occlusion can result in infarction of as much as 40% of the LV, it is highly plausible that larger ischemic injuries, similar to larger resection injury, are incompatible with complete regeneration [14].

Cryogenic injury

Cryogenic injury is another mouse model for studying heart regeneration and cellular remodeling. Unlike MI, the cryoinjury model induces confluent necrosis but does not spare cells within the center of the lesion. It can also have interdigitating viable and necrotic tissue [15]. Interestingly, cardiomyocytes are capable to proliferate sufficiently to effectuate myocardial regeneration after extensive cryoinjury of the LV myocardium in adult mice. High levels of macrophages precede and coincide with the highest levels of cardiomyocyte proliferation, suggesting a functional role of these cells in myocardial regeneration [16].

Cryogenic injury can be induced in several places throughout the heart, depending on the investigators’ area of interest. An open thoracotomy approach is used to induce cryoinjury on the LV, because this allows direct visualization of the freezing process and immediate validation of the resulting freeze-thaw injury. A liquid nitrogen-cooled aluminum probe is applied directly onto the anterior LV for ~10 seconds. The frozen myocardium thaws completely 10-15 seconds following probe removal, and the injured area exhibits a deep red coloration [12]. Three 1-min exposures cause an extensive lesion on the midventricular portion of the anteriolateral LV wall [11].

Another approach is through an abdominal incision to induce cryoinjury on the right ventricle (RV). Mice are anesthetized and the abdomen is opened through a transverse laparotomy. The translucent diaphragm is exposed by lifting the chest with a divaricator. The surface of the heart is now visible through the diaphragm and the desired area for the injury can be selected. A blunt tip stainless steel Von Graefe hook is directly placed on the diaphragm after cooling for ~2 min in liquid nitrogen to make an apical or midventricular RV injury. Two sequential 10-s exposures (with an intermittent 30-s interval) cause an extensive, transmural lesion on the lateroapical portion of the RV wall with an extension to the apical LV wall. A single 30-s exposure causes an extensive, transmural lesion on the midventricular RV wall with no extension to the septum [11]. This procedure has also been described in rats [17].

Doxorubicin (DOX)-induced HF

Nonischemic cardiomyopathy accounts for approximately one-third of HF cases. Unlike ischemic forms, which are amenable to palliative procedures like revascularization and remodeling operations, nonischemic cardiomyopathies, once they have reached an end stage of drug refractoriness, can only be treated radically by heart transplantation. DOX-treated mice receive a single dose of DOX at 15-20 mg/kg i.p., which causes clinical symptoms of HF [18]. Mice are studied five days later because at this time point more than 5 final half-lives of elimination of DOX from both plasma and cardiac tissue in mice has taken place and, at the time of functional and immunohistochemical assessment, DOX is no longer present in the blood or cardiac tissues. LV fractional shortening and cardiac output are significantly reduced (35 and 23%, respectively) relative to control animals. This murine model of severe LV dysfunction by DOX cardiotoxicity mimics the human pathology. Changes are consistent with clinical observations, suggesting that this murine model is appropriate for mechanistic evaluations [19].

Transgenic lines

One of the most advantageous aspects of utilizing mice is the ability to make genetic models. Although such models can be produced in larger species, mouse models can be developed in a shorter time period due to their short gestation age at a substantially lower cost [4].

Dilated cardiomyopathy (DCM)

Two strains of mice have been established to model the development, progression, and regression of DCM in humans. Muscle LIM protein (MLP) null mice are engineered by a deletion of MLP, an actin-associated cytoskeletal protein and Casequestrin (CQS) mice have a cardiac-restricted overexpression of the calcium binding protein CQS. Both produce a HF phenotype that replicates various aspects of human DCM. The MLP-/- mice appear to closely replicate human DCM, with progressive reduction in cardiac function beginning at 4-6 months, whereas CSQ mice develop rapid-onset DCM resulting in mortality by 9-14 weeks [20]. Echocardiography shows normal contractility in 4-week-old MLP null mice and a progressive decrease in contractility with increasing age. This suggests that contractile dysfunction is not the primary defect in this mouse model of DCM. Rather, the lack of MLP causes hypertrophy, resulting in HF with an increase in connecting tissue and increased myocardial stiffness [21].

Hypertrophic cardiomyopathy (HCM)

Mouse models in which cMyBP-C is homozygously ablated (cMyBP-C–/–) have been used to study the role of cMyBP-C in normal cardiac contractility and the development of HCM. Alterations in contractile kinetics serve as the primary pathophysiologic trigger for the development of hypertrophy in this model [22]. Another transgenic mouse model of HCM that overexpresses myotrophin in the heart, the Tg mouse, was also developed. This model showed hypertrophy as early as 4 weeks of age that progressively led to HF with severe compromised function. All the symptoms in this model mimic human HF. Tg mice demonstrated prevalent DNA damage in hearts during transition from long standing hypertrophy to HF and the induction of the cell regenerative machinery, especially in cardiac myocytes. Human HCM heart samples show similar changes in several genes for apoptosis and cell regeneration. Data in the Tg mouse model convincingly suggest that cell death and regeneration take place simultaneously during the transition of hypertrophy to HF [23].

Autoimmune cardiomyopathy (AICM)

To develop an animal model for AICM, DQ8 transgenic non obese diabetic (NOD) mice were crossed with a NOD Major Histochmpatibility Complex (MHC) class II β-chain knockout (KO) line. Animals from the original DQ8 transgenic NOD line developed spontaneous autoimmune diabetes at the same rate as the regular NOD/LtJ strain. However, when the DQ8-NOD animals were crossed with the NOD MHC class II KO line, the resulting DQ8+/+, IAβ–/– NOD animals no longer developed diabetes or insulitis; instead, they developed progressive DCM (hearts three to four times than normal size) and HF leading to premature death in both males and females. As the animals aged, first-degree atrioventricular (AV) block became widespread in the population, and by 18 weeks, the majority of animals had progressed to second degree or complete AV block. Histological examination of end-stage hearts showed pancarditis, with grossly dilated atria and only a few live cardiomyocytes remaining in mostly fibrotic atrial walls, which in places were paper thin [24].

Duchenne muscle dystrophy (DMD)

DMD is an X-linked neuromuscular disorder caused by a mutation in the dystrophin gene [25]. Although limb muscle weakness and loss of ambulation are usually the initial clinical signs of the disease, patients with DMD die from respiratory failure or HF. To improve lifespan and quality of life, progressive loss of contractile function in the heart also needs to be prevented or halted [26].

The genotypic murine model for DMD is the dystrophin-deficient Mdx mouse. The disease phenotype is milder in Mdx mice in contrast to human counterparts [25]. They have a normal life span and do not show significant muscle weakness or cardiac alterations nor any of the skeletal or cardiac muscle lesions that develop at late time points in DMD patients, such as fibrosis and muscular atrophy [27]. This is partially due to the compensatory effects of utrophin, a dystrophin homolog that can offset the loss of dystrophin in mice [26]. Another transgenic mouse was developed to better model the severe cardiac dysfunction in humans with DMD. In this double knock-out mouse, where both dystrophin and utrophin are absent, cardiac contractile function is severely affected at 8 weeks-of-age, displaying the classic pathophysiological hallmarks of end-stage human cardiac failure [28].

Several studies using dystrophin-deficient mice bred with other strains were conducted to clarify mechanisms leading to cardiomyopathy in DMD. Different therapies have been used to treat the symptoms of cardiomyopathy in DMD, but these agents cannot replace lost or damaged cardiomyocytes resulting from lack of fully functional dystrophin. Generation of new cardiomyocytes in dystrophin-deficient cardiac muscle from an endogenous population of cardiac stem cells suggests a potential mechanism that may be exploited to delay or prevent DCM in DMD [29]. Macrophages are necessary for repair of damage in the heart, as they stimulate new cardiomyocytes [29]. Also, IL-10 might be an important immune-modulator in dystrophic muscle. Cardiac inflammation induced by IL-10 ablation induced cardiac dysfunction and decreased LV function with LV and RV dilatation [30].

Atrial fibrillation (AF)

Cardiac-specific LKB1 KO mice are used to study AF and associated pathologies. 95% of KO mice developed AF by 12 weeks. They also demonstrated variable heart rhythm disorders including first- and second-degree AV block, bundle branch block, premature atrial and ventricular contractions, and atrial flutter. AF resulted in increased fibrosis, apoptosis, and disrupted ultrastructure [31]. KO mouse heart developed dilated atrial cardiomyopathy, which was vulnerable to electrical and structural remodeling. Atrial dilation, stretch, fibrosis, loss of muscle mass, cellular and matrix remodeling, and disruption of gap junctions are documented in this model. AF caused LV systolic dysfunction with depressed LV ejection fraction and clinical HF. It accurately represents human AF with characteristic structural and electrical remodeling in atria [31].Go to:

Rat

Ligation-induced myocardial infarction (MI)

The procedure to induce rat MI is very similar to the one described above for mice. Briefly, after rats are anaesthetized, intubated, and connected to a ventilator, a left thoracotomy is performed in the fourth intercostal space, the heart exposed, and the LAD artery ligated 2-3 mm from its origin between the pulmonary artery conus and the LA [32–34].

Overload-induced cardiac hypertrophy

One of the most common and successful surgical models to create pressure overload HF is constriction of the ascending aorta. This model is clinically relevant because of its slow but steady progression from compensated cardiac hypertrophy to the decompensated phase and, finally, to the HF stage. Ascending aortic constriction can be performed in two ways, either by using sutures or by application of metallic clips, which is less surgically complicated. In this procedure, one has to locate the supra-valvular ascending aorta and insert the clip around the aorta to obtain the desired level of constriction [35,36].

Diabetic cardiomyopathy (DbCM)

Little attention has been devoted to defining the initial response of myocardial tissue to a short period of hyperglycemia in terms of proliferative properties of myocytes and alterations of cardiac stem cell storage before the appearance of the cardiomyopathic phenotype. A detailed knowledge of changes occurring at the very beginning of diabetes, when cardiac electromechanical performance is still normal, may suggest appropriate therapeutic approaches aimed at preventing the development of mechanical dysfunction and arrhythmogenesis, which characterizes more advanced stages of DCM [37].

To create an animal model for diabetes, a single intra-peritoneal injection of streptozotocin (STZ, 60 mg/kg) is applied to rats. This is one of the most commonly used experimental models of diabetes. Rats exhibit hyperglycemia and hyperlipidemia coupled with hyperinsulinemia [38]. Ventricular dysfunction and marked structural damage appear 12 weeks after STZ treatment. The first detrimental effect of metabolic changes is a marked loss of ventricular mass, in the absence of cardiomyocyte hypertrophy or accumulation of extracellular matrix [37]. However in STZ-injected rats, it is difficult to definitively exclude the possibility that the pathogenesis is related, at least in part, to a permanent toxic action of STZ [39].

Transgenic lines

MI in hypertensive rats

Coronary occlusion in spontaneously hypertensive rats (SHR) presents many similarities to MI in humans and represents an adequate model for the investigation of the role and mechanisms of stem cell therapy [40]. Hypertension in SHR causes higher LV systolic pressure, blood pressure, and RV and LV weight compared with their normotensive counterparts. After MI, hypertension accelerates LV dilatation and haemodynamic alterations, indicating that this animal model presents many similarities to MI in humans [32,40].

Type II diabetic rats

A rat model of Type II diabetes is the Goto-Kakizaki (GK) rat. GK rats have a stable, inheritable form of Type II diabetes characterized by mild hyperglycemia and hyperinsulinemia, with no obesity, hypertension, or marked hyperlipidemia. Under normoxic conditions, cardiac function in the GK rat is indistinguishable from that of control rats. A contractile defect in both systolic and diastolic LV function can be elicited in the GK heart during even brief moderate hypoxia. Regardless the level of insulin, a high level of extracellular glucose is sufficient for development of contractile abnormalities in diabetic cardiomyocytes, which may explain the absence of these abnormalities in the GK rat model where the level of hyperglycaemia is mild. Contractile dysfunction in the GK heart has a primarily metabolic basis [39].

Another rat model is the JCR: LA-cp rat. It is a normotensive, hyperinsulinaemic model of Type II diabetes, which differs from the GK rat in being obese and markedly hyperlipidaemic. In addition, these rats develop early atherosclerotic lesions of major blood vessels and occlusive coronary thrombi at later stages of the disease [39].

Duchenne muscular dystrophy (DMD)

A new model of dystrophin-deficient rats was made by microinjecting a mixture of TALE nuclease mRNA for DMD in rat zygotes, allowing for the generation of two DMD/Mdx rat lines. The lesions in heart and skeletal muscle in this model closely mimics those observed in DMD patients. As observed in DMD patients but not in Mdx mice, fibrosis is severe in all skeletal muscle examined as well as in cardiac muscle of DMD/Mdx rats. Cardiac muscles are affected with necrosis and fibrosis and show signs of progressive DCM. Echocardiography showed significant concentric remodeling and alteration of diastolic function. These results indicate that DMD/Mdx rats represent a new, invaluable small animal model for pre-clinical research on DMD [27].Go to:

Rabbit

Spontaneous watanabe heritable hyperlipidemic myocardial infarction (WHHL-MI)

Rabbit myocardium shares more similarities with human myocardium than small rodent myocardium; therefore, rabbit genetic models, albeit expensive, can be used as a steppingstone to determine whether a particular study can be extended to humans and larger animal models. Although, other large species such as canine and sheep more closely resemble the human heart, the cost of acquiring and housing rabbits is still significantly lower, making them an attractive alternative to larger animal models for cardiac research. Differences between rabbit and human myocardium remain, possibly resulting in a particular study or therapeutic intervention having differential effects in rabbits and humans. For example, rabbits might not serve as the best animal model for studying the effects of exercise on the cardiovascular system mainly because its heart rate reserve is much less than that in human and large animal models, such as canines, which would serve as better animal models in such cases [4].

A rabbit spontaneous MI model could be obtained from the selective breeding descendants of coronary atherosclerosis-prone WHHL-MI rabbits. Because the coronary lumen area stenosis was enhanced in these rabbits, the incidence of spontaneous WHHL-MI was increased in proportion to the serial nearly-occluded coronary lesions. Coronary plaques with complicated lesions are the major determinant for MI in these rabbits. However, observations indicate that the mechanisms for MI in WHHL rabbits are different from those in humans [41].Go to:

Cats

Hypertrophic cardiomyopathy (HCM)

Spontaneous cardiac diseases similar or identical to those in humans are extremely common in companion animals and are vastly underutilized as models of human cardiac disease. HCM is currently the most common heart disease in cats, and its incidence appears to be increasing. As in humans, feline HCM is characterized by hypertrophy of the (LV, impaired diastolic filling, secondary left atrial enlargement is usually evident, and variable right heart enlargement or hypertrophy may develop over time. Arrhythmias are uncommon unless severe left atrial enlargement is present. In cats with HCM, congestive HF and arterial thromboembolism are common clinical manifestations [42].

A specific breed, the Maine Coon, has a genetic mutation that makes the breed prone to suffer from HCM. The prevalence of HCM in cats homozygous for this mutation is much higher than in cats heterozygous for the mutation. However, it is highly likely that other causes (genetic or not) are also responsible for the disease in the Maine Coon [43]. Individuals of this breed can be used as relevant animal models to study the development and pathophysiology of HCM.Go to:

Conclusions

Small animal models are commonly used in cardiovascular research because of their many advantages over large animal models (see Table 1). They have a short life span, allowing the investigators to follow the natural history of the disease at an accelerated pace. Also, the development of genetically modified models allows for rapid establishment of proof-of-principle that can later be extended into larger animal models. However, rodents have a few disadvantages as well. They are phylogenetically very distant from humans and some pathophysiological features of disease and their response to pharmacological treatments may not be reliable predictors for humans. Thus, translational aspects and value of genetic small animal models must be interpreted with caution. Although they may display some of the characteristics of human cardiac disease, they typically do not recapitulate all aspects of it.

Table 1

Advantages and disadvantages of small animal models

AdvantagesDisadvantages
• Lower maintenance costs• Phylogenetically distant from humans
• Easier to handle and house• Pathophysiology of disease may not be translatable to humans
• Shorter gestation time and lifespan• Different response to pharmaceutics
• Suitable for proof-of-concept and “high-throughput” studies• Not suitable for chronic studies
• ischemia-reperfusion induced arrhythmias are infrequent and easy to reverse when they occur
• Suitable for genetic selection and production of transgenic strains

Determining the best experimental model of a human condition requires a number of decisions and compromises – especially in relation to obtaining the optimal balance between the quantity and quality of the data produced vs the relevance of the data to the condition under investigation. In assessing the utility of an investigative model, it is necessary to identify the research objectives. The question under study will greatly influence the choice of the most appropriate model and determination of cardiac function and malfunction. It is also important to standardize the procedures used in order to obtain relevant and reproducible results that can be compared with other findings. Table 2 summarizes the most relevant small animal models for cardiac disease and whether the disease is artificially induced or spontaneous. This provides a basis in which to decide what model best suits a specific investigation.

Table 2

Relevant small animal models for cardiac disease (only one reference is listed for each model. See the Main text for more references)

AnimalsInduced modelsSpontaneous models
MouseCryoinjury [11]AF [31]
DOX induced HF [18]AICM [24]
MI [8]DCM [21]
DMD [28]
HCM [23]
RatCardiac hypertrophy [35] Cryoinjury [17]Hypertensive rat [40] DbCM [39]
DbCM [38]DMD [27]
MI [33]
RabbitsWHHL-MI rabbits [41]
CatsHCM [42]

Abbreviations: AF: Atrial fibrillation, AICM: Autoimmune cardiomyopathy, DbCM: Diabetic cardiomyopathy, DCM: Dilated cardiomyopathy, DMD: Duchenne muscular dystrophy, DOX: Doxorubicin, HF: Heart failure, HCM: Hypertrophic cardiomyopathy, MI: Myocardial infarction, WHHL-MI: Watanabe heritable hyperlipidemic myocardial infarction.Go to:

Acknowledgements

We thank Dr. Janet Webster of the Fralin Life Science Institute at Virginia Tech for providing a review of the manuscript. This work was supported by the Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech #JFC2014_JIAHE958451025). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.Go to:

Disclosure of conflict of interest

None.Go to:

Abbreviations

AFatrial fibrillationAICMautoimmune cardiomyopathyAVatrioventricularCSQcalsequestrinDbCMdiabetic cardiomyopathyDCMdilated cardiomyopathyDMDDuchenne muscular dystrophyDOXdoxorubicinGKGoto-KakizakiHCMhypertrophic cardiomyopathyHFheart failureKOknockoutLADleft anterior descendingLVleft ventricleMHCmajor histocompatibility complexMImyocardial infarctionMLPmuscle LIM proteinNODnon obese diabeticRVrights ventricleSHRspontaneously hypertensive ratSTZstreptozotocinWHHL-MISpontaneous Watanabe heritable hyperlipidemic myocardial infarctionGo to:

References

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Curr Opin Pharmacol. 2010 Oct;10(5):571-7. doi: 10.1016/j.coph.2010.06.003. Epub 2010 Jul 1.

Modeling metastasis in the mouse.

Bos PD1, Nguyen DX, Massagué J.

Author information

1Cancer Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.

Abstract

Metastasis is a complex clinical and biological problem presently under intense study, and several model systems are in use to experimentally recapitulate and dissect the various steps of the metastatic process. Genetically engineered mouse models provide faithful renditions of events in tumor progression, angiogenesis, and local invasion that set the stage for metastasis, whereas engrafting of human or mouse tumor tissues into mouse hosts has been successfully exploited to investigate metastatic dissemination and colonization of distant organs. Real-time, high-resolution microscopy in live animals, and comprehensive genetic and molecular profiling are effective tools to interrogate diverse metastatic cancer cell phenotypes as well as the metastatic tumor microenvironment in different organs. By integrating the information obtained with these complementary approaches the field is currently obtaining an unprecedented level of understanding of the biology, molecular basis, and therapeutic vulnerabilities of metastasis.

Copyright © 2010 Elsevier Ltd. All rights reserved.PMID: 20598638 PMCID: PMC2952835 DOI: 10.1016/j.coph.2010.06.003[Indexed for MEDLINE] Free PMC Article

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An opinion may be supported by facts and principles, in which case it becomes an argument.´´ https://en.wikipedia.org/wiki/Opinion @ ´´Harvard was established in 1636 by vote of the Great and General Court of the Massachusetts Bay Colony.´´ https://en.wikipedia.org/wiki/Harvard_University @ OTHER VERY IMPORTANT INFORMATION @ www.wordpress.com www.wikipedia.org www.nih.gov www.ucla.edu www.stanford.edu www.darpa.mil www.whitehouse.gov www.usa.gov www.nature.com www.nejm.com www.google.com www.gmail.com www.yahoo.com www.youtube.com www.linkedin.com www.facebook.com www.twitter.com www.instagram.com www.cnn.com www.bbc.com www.reuters.com www.bloomberg.com www.nasdaq.com www.wellsfargo.com www.harvard.edu www.mit.edu www.nasa.gov www.caltech.edu www.yale.edu www.columbia.edu www.duke.edu www.princeton.edu www.nobelprize.org www.famerp.br www.uftm.edu.br www.timeshighereducation.com www.microsoft.com www.spacex.com www.tesla.com www.alibaba.com www.amazon.com www.abc.com www.ft.com www.exame.com www.forbes.com www.time.com www.cdc.gov www.genome.gov www.fda.gov www.pfizer.com www.cornell.edu www.nyu.edu www.washingtonpost.com www.stripe.com www.worldbank.org www.proton.me www.fbi.gov www.cia.gov @ * Name of People -> Place of Work - Time/Data - Internet/Time/Subject/Internet Pioneers/Work/Life (Probability of this Fact - Probabilidade deste Fato) https://science1984.wordpress.com/2023/11/22/no-one-informed-me-by-email-for-example-that-they-used-or-are-using-as-references-the-graphics-i-made-of-my-dissertation-these-graphics-are-not-included-in-the-related-scientific-article-in-scie/ – So Many Positive Feedbacks about this Blog by Facebook, LinkedIn and Twitter – This Blog Links -> https://science1984.wordpress.com/2023/10/15/so-many-positive-feedbacks-about-this-blog-by-facebook-linkedin-and-twitter/ ´´Thank you so much for sharing´´ https://www.linkedin.com/in/hosna-salmani-a016a5125/ ´´I would also like to compliment you on your excellent writing skills. Do you do speaking engagements?´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´I have a colleague I would like to refer you to.´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´Very impressive work you have done. You appear to be well on your way in a rewarding career. What inspired you to take this path?´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´This is amazing work you are doing. Let me look into my network and see who might be best to refer you to. Very impressive what I see you have done so far. My son does financial writing and maybe he might have more connections.´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´Thank you. I am interested to learn if you have a good grasp on health economics from the consumer side and also from the side of health system payers or health plans globally. Is this part of your expertise?´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´I am a Preceptor for masters in public health student.´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´Interesting´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´I am also involved in https://healthspanaction.org/ ´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´Let me know if you are interested in joining. It is a global effort.´´ https://www.linkedin.com/in/bill-remak-111a85a/ ´´Thanks.´´ https://www.linkedin.com/in/bill-remak-111a85a/ https://www.facebook.com/Bioquantumacademy ´´Thank you for sharing. Wonderful work done. I will definitely visit your blog often.´´ https://www.facebook.com/Bioquantumacademy - For a better world always in all ways! - Por um mundo melhor sempre de todas as formas - 10/14/2023 - I am available to receive partnership proposals with this Blog. Estou disponível para receber propostas de parceria com este Blog. #ThisBlogPost #ThisBlogIntentions #Names #Times #IndeterminedTime #Probabilities #Countries #HighVisibility #VeryHardWork #WorldHistory #Information #Knowledge #DNA #Cell #Person #Ages #People #Age #ScientificDiscoveries #Research #Projects #HumanLongevity @ My Email Accounts: rodrigoncal1984@gmail.com rodrigonunescal@yahoo.com rodrigoncal1984@yahoo.com rodrigonunescal1984@proton.me & Maybe I can earn money with this blog by a partnership like I do a post about the possible partner because of the high visibility of my blog around the world. Many people from different countries have visited it, including excellent professors, scientists and/or researchers worldwide. Maybe a partner have interest in paying money to me monthly for it. I am not sure about this subject. It is the first time I do it. I do not have any experience about it. The most important fact is that this blog content is excellent with a very high level of importance to the world progress always! As you know, the human expectancy of life needs to increase so much faster more and more by very efficient research and projects always! #probability #reader #leitor #people #pessoas #possibilidade #economy #memory #memória #economia #dinheiro #money #países #countries #cidades #city #Internet  A very important fact is that many people from diferent countries visit it daily. Other ´´thing´´ is that excellent professors, scientists and/or researchers from the world have visited it. Its goal is contribute significantly to the world progress always, like increasing the human expectancy of life faster, more and more. - My intentions have always been, are and will always be to help people from different countries in some way because this Blog content is excellent and always has a high level of importance for the world progress! As you know, everyone has their own way of thinking and always acting. For a better world in all ways always! Minhas intenções sempre foram, são e sempre serão de ajudar de alguma maneira as pessoas de diferentes países porque o conteúdo deste Blog está excelente com um alto nível de importância para o progresso mundial sempre! Como você sabe, cada um tem sua própria maneira de pensar e agir sempre. Por um mundo melhor sempre de todas as formas! https://science1984.wordpress.com/2023/11/21/my-intentions-have-always-been-are-and-will-always-be-to-help-people-from-different-countries-in-some-way-because-this-blog-content-is-excellent-and-always-has-a-high-level-of-importance-for-the-wo/ * There are very important posts in my blog and the number of the posts is very high. My intention was always do my best in this blog to the world. http://www.darpa.mil http://www.ucla.edu - Supreme Court of the United States -> http://www.stanford.edu https://www.supremecourt.gov/ – 10/07/2023 – Probability->Money->Each email address in the email lists I did if the person agree->Money to me (I did it)->Recognition of the very hard work to do it, my idea about it and the level of importance of the email lists to the world progress always in several aspects -> Supreme Court of the United States – https://www.supremecourt.gov/ @ 10/07/2023 —> Very Important Phrases from Bill Gates, Jeff Bezos, Elon Musk and Steve Jobs – YouTube Videos @ –> Frases Muito Importantes/Reading/Leitura/Click/This Post/Este Post -Probability/Possibilidade(s) – Change of Opinions/Mudança de Opiniões – Vida(s) Humana(s) /Human Life / Human Lives – True Facts/Fatos Verdadeiros/Internet – Pessoa(s)/Person/People – Tempo(s) de Vida/Time of Life/Idade(s) /Age/Ages – Respect/Respeito @ ´´Se você quer chegar onde a maioria não chega, faça o que a maioria não faz.´´ Bill Gates / ´´A chave do sucesso nos negócios é perceber aonde o mundo se dirige e chegar ali primeiro.´´ Bill Gates / ´´O poder não vem do conhecimento, e sim do conhecimento compartilhado.´´ Bill Gates Bill Gates / ´´As pessoas sempre têm medo das mudanças. Tinham medo da eletricidade quando foi inventada.´´ Bill Gates / ´´Sem livros, sem leitura, os nossos filhos serão incapazes de escrever – inclusive sua própria história.´´ Bill Gates / “Você precisa estar disposto a ser incompreendido se você for inovar.” Jeff Bezos / “Se você não quiser ser criticado, nunca faça nada.” Jeff Bezos / “Detalhes importam, vale a pena esperar e fazê-los direito”. Steve Jobs / “A melhor maneira de escapar de uma caixa é inventando seu próprio caminho para fora”. Jeff Bezos / “Você quer passar o resto de sua vida vendendo água com açúcar, ou você quer uma chance de mudar o mundo?” Steve Jobs / “As pessoas que estão mais frequentemente corretas são aquelas que mais frequentemente mudam de opinião.” Jeff Bezos / “Não deixe a opinião dos outros abafar sua voz interior”. Steve Jobs / “Se você dobrar a quantidade de experimentos que faz por dia, estará dobrando sua inventividade.” Jeff Bezos / “O que é perigoso é não evoluir”. Jeff Bezos / “Trabalhe duro. Divirta-se. Faça história.” Jeff Bezos / “Os fazedores são os maiores pensadores. As pessoas que criam e mudam a indústria são pensadores e fazedores em uma só pessoa”. Steve Jobs / “Eu quero colocar uma marca no universo”. Steve Jobs / “A única maneira de fazer algo excelente é amar o que você faz. Se você ainda não a encontrou, continue procurando. Não se acomode”. Steve Jobs / “Você pode encarar um erro como uma besteira a ser esquecida, ou como um resultado que aponta uma nova direção”. Steve Jobs / “Reclamar não é uma estratégia. Temos que lidar com o mundo tal como ele é, e não tal como gostaríamos que ele fosse.” Jeff Bezos / “Acho que esse é o melhor conselho de todos: pense constantemente sobre como você pode fazer as coisas melhor e questione a si mesmo.” — Elon Musk https://science1984.wordpress.com/2023/10/21/very-important-phrases-from-bill-gates-jeff-bezos-elon-musk-and-steve-jobs-youtube-videos-frases-muito-importantes-reading-leitura-click-this-post-este-post-probability-pos/ https://science1984.wordpress.com/2023/10/14/i-am-available-to-receive-partnership-proposals-with-this-blog-estou-disponivel-para-receber-propostas-de-parceria-com-este-blog-thisblogpost-name-indeterminedtime-time-probabilities-countries/ – 10/07/2023 – The graphics I did about ´´my´´ dissertation and ´´my´´ monograph that can be excellent references for many types of future research and projects, of course. Os gráficos que fiz sobre ´´minha´´ dissertação e ´´minha´´ monografia podem ser ótimas referências para futuras pesquisas e projetos de vários tipos, com certeza. https://science1984.wordpress.com/2022/12/13/65792/ – Internet – Gene – 100 gene – 100 – gene(s) – No Gene – No(s) Gene(s) - No Internet – 100 – Sem Internet – Vida(s) Humana(s) – Human Life – Grau(s) de Influência(s) – Level of Influence – No Communication – Sem Comunicação – Internet History – Test – Teste(s) – Internet Pioneer – UCLA/Stanford – Internet Pioneers – Pioneiro(s) da Internet – País(es) – USA – Time/Tempo – Country – World – Countries – City – Cities – Person (Pessoa) – Other Person (Outra Pessoa) – Distance – Distância(s) – World – History – World History – Internet Society – Society – Times – Time(s) – Tempo(s) – Human Lives – Human Memory – Memória(s) Humana(s) – Futuro – Future – Now – Agora – Passado – Past – Presente – Present – Idade(s) – Age – Ages – Human Longevity – Longevidade Humana https://science1984.wordpress.com/2018/12/20/15362/ -> "Service to mankind, broadly or individually, is the greatest fulfillment in life" The Foundation's Founder, Nobel Laureate, Dr. George H. Hitchings - I´ve graduated in Biomedicina at Federal University of Triangulo Mineiro in Chagas disease in laboratory (Uberaba – 2003-2007) and I have a Master´s degree at Faculty of Medicine of Sao Jose do Rio Preto (2008-2012) in lung cancer research in mice. Nowadays I work as inspector of students since 2012 in Sao Jose do Rio Preto, Brazil. (09/17/2023) https://science1984.wordpress.com/2023/03/18/77283/ -> Share this Blog and this Blog Posts to your contacts and inform them to do the same and so on, if possible!! @ I – EU – Internet – Internet Society – World History – Internet History – Probability – World Progress – Age – Ages – Human Longevity https://science1984.wordpress.com/2023/04/04/protecao-mundial-sempre-a-favor-do-bem-worldwide-protection-always-in-favor-of-good-https-science1984-wordpress-com-2023-04-04-physicalimmortalityaccordingtoraykurzweil-veryhighcoincidence-prob/ – 9 equations that changed the world – By Adam Mann last updated July 28, 2022 For a better world always in all ways! Por um mundo sempre melhor de todas as formas! @ #probabilities #person #people #lives #age #ages #life #countries #cities #city #country #internet https://science1984.wordpress.com/2023/01/08/goals-of-this-blog-emails-reading-leitura-probability-usa-authorities-team-times-tempos-memory-law-leis-energy-he/ Objectives of this Blog -@? E-mails – Reading – Leitura – Probability – USA – Authorities – Team – Times – Tempo(s) – Memory – Law – Lei(s) – Energy – Health – Life – Vida(s) – Feelings – Time(s) @ Positive Feedbacks about this Blog & Sharing interesting and important information is a very important act because it helps in the diffusion of them in the world. Of course, it has to be true and not fake news. #probabilities #probabilidades #Internet https://science1984.wordpress.com/2021/09/04/do-the-downloads-share-the-diffusion-of-very-important-information-and-knowledge-is-essential-for-the-world-progress-always-thanks/ -> The diffusion of relevant information and knowledge in this Blog is essential for the world progress always in many aspects, of course! @ EU – Countries – I – Internet Pioneer – Team – Internet Society – Society – Time – Times – Probabilities – True Facts – Person – People @ #reader #ThisBlog #Science #Technologies #Innovations #economy #cities #images #data #links #websites #SocialNetworks #videos #texts #photos #prices #money #clicks #vision #communication #HumanLongevity @ https://science1984.wordpress.com/2023/01/08/se-alguem-quiser-usar-as-listas-de-e-mails-que-fiz-em-2022-a-pessoa-tera-que-enviar-e-mail-para-os-seguintes-enderecos-de-e-mails-chancellorucla-edu-presidentwhitehouse-gov-vice_presidentw/ www.whitehouse.gov www.ucla.edu www.darpa.mil www.stanford.edu www.usa.gov https://science1984.wordpress.com/2023/04/06/scientific-community-physicalimmortalityproject-veryhighmoneyinvolved-extensiveemaillistsidid-graphics-data-veryhighutilitiesinthisproject-investigations-logicalfacts-law-justice-fbi-cia/ Ray Kurzweil’s predictions are 86% accurate &@ Probability (0 or not 0)->Person/People->Money->Me->My Idea->I->Work (Recognition)->Use of the Email Lists and/or the Graphics I did (´´my´´Dissertation and/or ´´my´´ Monograph) -> Human Physical Immortality Projects and Research of the World and/or Other Scientific Projects and Research Worldwide Subject (Assunto) @ Vida/Life – É muito importante combater pessoas maldosas e muito maldosas até nos pensamentos. #Memory #Memória – Past/Present/Future # Passado/Presente/Futuro https://science1984.wordpress.com/2022/11/12/subject-assunto-vida-life/ https://science1984.wordpress.com/2023/04/04/physicalimmortalityaccordingtoraykurzweil-veryhighcoincidence-probability-time-extensiveemaillistsidid-gmail-emails-archive-database-google-yahoo-system-pioneer-thisblogposts-recognitio/ @ www.google.com www.gmail.com www.time.com www.yahoo.com www.youtube.com https://web.whatsapp.com/ www.twitter.com www.facebook.com www.linkedin.com www.nasdaq.com www.nih.gov www.timeshighereducation.com www.fda.gov www.cdc.gov www.wellsfargo.com www.worldbank.org www.fbi.gov www.cia.gov www.pfizer.com www.harvard.edu www.mit.edu www.ucla.edu www.usa.gov www.whitehouse.gov www.nasa.gov www.stanford.edu www.apple.com www.microsoft.com www.spacex.com www.tesla.com www.facebook.com/scientificblog www.ft.com www.nyt.com www.wsj.com www.reuters.com www.washingtonpost.com www.cnn.com www.bbc.com www.abc.com www.forbes.com www.wikipedia.org www.stripe.com www.wordpress.com www.nobelprize.org www.columbia.edu www.yale.edu www.duke.edu www.cornell.edu www.nyu.edu www.famerp.br www.uftm.edu.br www.ufscar.br www.uel.br www.pucsp.br www.ita.br www.embraer.com www.nobelprize.org www.nejm.com www.nature.comhttps://web.whatsapp.com/@ www.google.com www.gmail.com www.time.com www.yahoo.com www.youtube.com www.twitter.com www.facebook.com www.linkedin.com www.nasdaq.com www.nih.gov www.fda.gov www.cdc.gov www.wellsfargo.com www.worldbank.org www.pfizer.com www.harvard.edu www.mit.edu www.ucla.edu www.usa.gov www.whitehouse.gov www.nasa.gov www.stanford.edu www.apple.com www.microsoft.com www.spacex.com www.tesla.com www.facebook.com/scientificblog www.ft.com www.nyt.com www.wsj.com www.reuters.com www.washingtonpost.com www.cnn.com www.bbc.com www.abc.com www.forbes.com www.wikipedia.org www.stripe.com www.wordpress.com www.nobelprize.org www.columbia.edu www.yale.edu www.duke.edu www.cornell.edu www.nyu.edu www.famerp.br www.uftm.edu.br www.ufscar.br www.uel.br www.pucsp.br www.ita.br www.embraer.com www.nobelprize.org www.nejm.com www.nature.com – Unfortunately there are in the world fatal diseases, rare diseases and unknown diseases https://science1984.wordpress.com/2022/09/14/unfortunately-there-are-fatal-diseases-and-or-unknown-diseases/ World´s Protection in All Ways Now and Always!! @ Proteção Mundial de Todas As Formas Agora e Sempre!! https://science1984.wordpress.com/2022/09/02/worlds-protection-in-all-ways-and-aspects-always-protecao-do-mundo-em-todos-os-sentidos-e-aspectos-sempre/ https://science1984.wordpress.com/2022/08/14/61244/ Sempre serei do bem. I´ll always be a good person. @ World History – História Mundial – 100 Genes – No Gene/Sem gene – Numbers/DNA -Read/Listen/Speak/Type/Write – Country https://science1984.wordpress.com/2022/09/16/human-dna-human-brain-human-cell-extraordinary-skills-work-the-mentalist-number-1-of-the-world-mind-reading-reading-of-minds-other-person-people-thinking-thoughts-visit-an/ Subject @ Vida/Life – It is very important to fight evil and very evil people, even in your thoughts. #Memory #Memory – Past/Present/Future # Passado/Presente/Futuro https://science1984.wordpress.com/2022/09/17/protection-of-children-adolescents-women-and-elderly-people-now-and-always-protecao-de-criancas-adolescentes-mulheres-e-de-pessoas-idosas-agora-e-sempre/ – 09/17/22 – Protection of children, women, adolescents and elderly people now and always!! Proteção de crianças, mulheres, adolescentes e de idosos agora e sempre!! @ Sharing interesting and important information is a very important act because it helps in the diffusion of them in the world. Of course, it has to be true and not fake news. https://science1984.wordpress.com/2022/09/17/unfortunately-there-are-in-brazil-and-other-countries-many-very-bad-people-the-world-always-needs-to-improve-quickly-in-the-best-way-for-people-to-live-better-and-longer-infelizmente-existem-no-br/ 09/17/2022 – Unfortunately there are in Brazil and other countries many very bad people. The world always needs to improve quickly in the best way for people to live better and longer. @ Infelizmente existem no Brasil e em outros países muita gente muito ruim. O mundo sempre precisa melhorar rapidamente da melhor forma para que as pessoas vivam melhor e por mais tempo. @ Sharing interesting and important information is a very important act because it helps in the diffusion of them in the world. Of course, it has to be true and not fake news. https://science1984.wordpress.com/2022/10/22/my-intentions-were-and-always-will-be-to-help-people-in-some-way-for-a-better-world-minhas-intencoes-foram-e-sempre-serao-de-ajudar-de-alguma-forma-as-pessoas-por-um-mundo-melhor-probability-r/ - My intentions were and always will be to help people in some way for a better world. Minhas intenções foram e sempre serão de ajudar de alguma forma as pessoas por um mundo melhor.    https://science1984.wordpress.com/2022/07/02/60219/ – Physical Immortality ? There´re people linked to Scientific Community who believe in it in the future. This is a very important subject in this post, of course. https://science1984.wordpress.com/2021/11/27/world-scientific-community-detection-unknown-diseases-fatal-diseases-other-diseases-age-times-time-ages-person-birth-death-same-age-analysis-graphics-projects-scientifi/ – World Scientific Community – Detection – Unknown Diseases – Fatal Diseases – Other Diseases – Age – Times – Time – Ages – Person – Birth – Age of Death – Same Age – Same Ages – Facts – Time – Analysis – Different and Detailed Graphics – Physical Immortality (?) – Different Tests – Projects – Scientific Researches – Mice Researches – Human Researches – Animal Testing – DNA – Cell – RNA – New Drugs – New Vaccines – New Therapeutical Substances – Personalized Medicine – Details – People – Genetics – Physiology – Biochemistry – Molecular Biology – Biological Factors – Human Body – New Prevention Methods – New Diagnosis Mehods – New Prognosis Methods – New Treatments Mehods – New Medical Devices – Different Graphics – New Tests – New Susbstances – New Analysis – Future – Control Groups – Study Groups – Graphics Comparisons – p Values – Probabilities – Statistics – Biostatistics – Very High Efficiencies – Money – Price – Pharmacology – Parasitology – Cardiology – Neurology – Subjects – New Researches – Very Innovative Graphics – Science Data – New References – Scientific Journals – Dissertations – Monographs – Articles – Impact Factor – Science – Different Technologies – Investments – Politics – Laboratories – Industries – Top Universities – Top Research Centers – Researchers – Scientists – Professors – Doctors – City – Cities – Internet – Internet Society – Internet History – History – World History – Investigation – YouTube Videos (Internet Pionners) – Lives – Thinking – Contribuitions – World – Energy – Intentions – Objectives – Goal – Blog – Work – Improvement – Health – Mind – Human Life – Information – Knowledge – New Projects – New Objectives – Team – Different Departments – Innovations – Different Communication – Respect – Television – Radio – Journals – Magazines – Write – Type – Listen – Speak – Read https://science1984.wordpress.com/2021/01/10/40820/ – ´´Understanding the great difficulties of a given subject can be a very long process, however, of great value for the progress of Science in all aspects´´ Rodrigo Nunes Cal – 01/10/2021 @ ´´Compreender as grandes dificuldades de um determinado assunto pode ser um processo muito demorado, porém, de grande valia para o progresso da Ciência em todos os aspectos´´ Rodrigo Nunes Cal – 10/01/2021 @ Future pandemic preparedness, powered by genomics – Next-generation sequencing is helping to steer the global response to SARS-CoV-2, and will help the world to prepare for future pandemics – Produced by Nature Research Custom Media Illumina – © 2021 Springer Nature Limited @ Mysterious ‘kick’ just after the Big Bang may have created dark – By Paul Sutter – Astrophysicist @ Images about this blog stats and other important images & Internet – Images – Texts – Data – Reading – Thinking – Time – Person – People – Statistics – World – Countries – Cities – Thinking – Thoughts – Intentions – Influences – Times – Memories – MEMORY – Pesquisa – Research – Researches – References -Graphics – Dissertation – Monograph – Innovations – Human Longevity – Internet Society – Society – Internet History – History – Internet ´´The world people need to have very efficient scientific research and projects resulting in very innovative drugs, vaccines, therapeutical substances, medical devices and other technologies according to the age, the genetics and medical records of the person. So, the treatment, diagnosis and prognosis will be very efficient and better, of course´´. Rodrigo Nunes Cal ´´É muito importante, com certeza absoluta, prevenir e detectar crimes de abuso sexual contra crianças e adolescentes e demais crimes no mundo digital e no mundo sem o uso da Internet´´. Rodrigo Nunes Cal ´´It´s very important, with absolute certainty, to prevent and detect crimes of sexual abuse against children and adolescents and other crimes in the digital world and in the world without the use of the Internet´´. Rodrigo Nunes Cal ´´Sex education is very important for sure´´. Rodrigo Nunes Cal ´´A educação sexual é muito importante, com certeza absoluta´´. Rodrigo Nunes Cal ´´It is very important to try to improve the society we live in in some way, at least on some issue. Sometimes there is no success in this attempt, but the important thing is to leave a very good example for other people to follow the same path in trying to solve various types of problems present in today’s world, in which these problems negatively influence people’s lives. somewhat reducing the life span of people in different countries around the world´´. Rodrigo Nunes Cal ´´É muito importante tentar melhorar a sociedade em que vivemos de alguma forma em algum assunto, pelo menos. Às vezes não há êxito nesta tentativa, mas o importante é deixar um exemplo muito bom para as outras pessoas seguirem no mesmo caminho na tentativa de resolução de problemas de vários tipos presentes no mundo de hoje, nos quais estes problemas influenciam negativamente na vida das pessoas diminuindo de certa forma o tempo de vida das pessoas nos diferentes países do mundo´´. Rodrigo Nunes Cal – ´´What happens in the digital world does not always happen in human life without the internet, obviously ´´. Rodrigo Nunes Cal The graphics I did about variations of all mice weights during all experimental time are not in the article of my dissertation nor in my dissertation. The details of exercise and rest times of the animals aren´t in the article: The influence of physical activity in the progression of experimental lung cancer in mice – Pathol Res Pract. 2012 Jul 15;208(7):377-81. Renato Batista Paceli 1, Rodrigo Nunes Cal, Carlos Henrique Ferreira dos Santos, José Antonio Cordeiro, Cassiano Merussi Neiva, Kazuo Kawano Nagamine, Patrícia Maluf Cury – https://pubmed.ncbi.nlm.nih.gov/22683274/ These graphics I did of ´´my´´ dissertation that are related to all animal ages in each group including control groups can be an excellent reference for future researches in animal testing and in human researches in the area of genetic engineering for example. These data are in my blog as well as the many direct invitations that I´ve received to participate in relevant scientific events of the world in a very little time. The age of the humans, animals and the genetics, physiology, biochemistry related to them influence directly in determined ways in human expectancy of life. ´´More than 800 researchers have added their names as signatories. A series of related articles is being published by the American Statistical Association this week (R. L. Wasserstein et al. Am. Stat. https://doi.org/10.1080/00031305.2019.1583913; 2019). “The tool has become the tyrant,” laments one article. https://www.nature.com/articles/d41586-019-00874-8 https://www.nature.com/articles/d41586-019-00857-9 American Statistical Association – https://www.amstat.org/ ´´We propose to change the default P-value threshold for statistical significance from 0.05 to 0.005 for claims of new discoveries.´´ https://www.nature.com/articles/s41562-017-0189-z Published: 01 September 2017 Daniel J. Benjamin, James O. Berger, […]Valen E. Johnson Nature Human Behaviour volume 2, pages6–10 (2018) – What levels of influence does the Internet have on people’s lives in its various aspects? The fact is that many situations that occurred and occur daily in the Internet environment would never occur in these people’s lives, due to several interconnected factors, since this virtual world is very complex and dynamic and is very linked to imagination and assumptions, for example. Depending on the circumstance and other factors, a lot of information that one person typed for another, he would not speak or read what he typed. Obviously the Internet has its very good and important side in people’s lives in many aspects. However, it has its negative and dark side in several interconnected issues, coming to be dangerous in the lives of many people, unfortunately. The Internet has two sides of the coin, one positive and one negative. Furthermore, as many of you already know, there is a huge amount of money directly and/or indirectly related to the Internet, which is strongly linked to the economy of a certain place. As you already know, the Internet originated from the great ideas and thoughts of people and since then, a lot has changed and has been changing constantly, causing each time another significant increase in the number of Internet users in various parts of the world. There are several types of influences in different intensities that the Internet can cause in people’s lives, depending, for example, on the types of website content that people access and their frequency of access, as well as there are different means of communication, such as the writing, speaking, writing something, and others. Certainly, there are sites that are much better than others in various subjects, causing a different type of behavior and view of the Internet user on this site. As many say, there is a virtual world very different from the real world. Obviously, the different technologies developed by people have impacts of different degrees on people’s lives, bringing different types of consequences, for example, very constructive and very negative and dangerous. Another very obvious thing is that not always what happened and/or happens in the present in the virtual environment happened and/or will happen in a personal contact between 2 people in certain circumstances, for example. Therefore, it is necessary to be very careful when browsing the Internet, as there are a lot of sites of different types, some much better than others on the same topic, causing different degrees of influence on people’s minds and actions at different time intervals, depending a lot on the usage time of a certain website, for example. Much Health and Peace for You Always!! #probability #reader #time #pioneer #USA #pioneers #ideas #people #person #thoughts #thinking #memory #tempo #probabilities #website #videos #links #websites #video #photo #images #photos #names #name #DifferentInfluences #HumanMind #Feelings #intentions #goal #objectives #tests #work #Internet #HumanInterdependence #test #life #speak #sincerity #virtual #real #HumanMinds #actions #life #health #probabilities #virtuality #reality #countries #economy #world @ YouTubeVideo – @ Who invented the Internet? And Why? Quem inventou a internet? E por que? https://www.youtube.com/watch?v=21eFwbb48sE www.youtube.com & https://www.youtube.com/watch?v=KF6sLCeBj0s Como a internet funciona? – Glad You Asked T1 – How does the Internet work? https://science1984.wordpress.com/2021/12/25/what-levels-of-influence-does-the-internet-have-on-peoples-lives-in-its-various-aspects-the-fact-is-that-many-situations-that-occurred-and-occur-daily-in-the-internet-environment-would-never-occur/ * www.fbi.gov www.cia.gov www.whitehouse.gov www.usa.gov www.facebook.com www.linkedin.com www.gmail.com www.yahoo.com www.proton.me Observação muito importante: Não fui eu quem disponibilizou a lista de e-mail que criei em 2015 para qualquer pessoa acessar na Internet em um link que já especifiquei para autoridades governamentais e policiais dos EUA pelo LinkedIn e Facebook (já mencionei esse assunto na minha conta de e-mail). No meu blog há detalhes sobre esta lista de e-mail que se refere a pesquisadores, cientistas e/ou cientistas do mundo que trabalharam (trabalham) nas 20 melhores Instituições de Ensino e Pesquisa do Mundo em 2015. Recebi muitos feedbacks positivos de excelentes profissionais da área de pesquisa e ensino que estão no post do meu blog, onde há explicações sobre os fatos envolvidos neste projeto, que foi enviado em formato PDF para cada um deles. A Comunidade Científica Mundial precisa obviamente de muito mais recursos de vários tipos para melhorar ainda mais os projetos científicos e a investigação, a fim de aumentar ainda mais a esperança de vida humana. Very important note: I was not the one who made the email list I created in 2015 available for anyone to access on the Internet in a link I have already specified to US government and law enforcement authorities via LinkedIn and Facebook (I have already mentioned this subject in my email account). email). On my blog there are details about this email list that refers to researchers, scientists and/or scientists in the world who worked (work) in the 20 best Teaching and Research Institutions in the World in 2015. I received a lot of positive feedback from excellent professionals from the area of ​​research and teaching that are in my blog post, where there are explanations about the facts involved in this project, which was sent in PDF format to each of them. The World Scientific Community obviously needs many more resources of various types to further improve scientific projects and research in order to further increase human life expectancy. – The Era of Modernity – Text typed by me on 09/18/2021. @ Very important links to download eBooks for free – Links muito importantes para baixar e-Books gratuitamente @ Relevant information related to book reading and its interconnected aspects in the school environment – ​​Rodrigo Nunes Cal – Part 1 and 2 (Documents) – Informações relevantes relacionadas à leitura de livros e seus aspectos interligados no ambiente escolar – Rodrigo Nunes Cal – Parte 1 e 2 (Documentos – Very important links to download eBooks for free – Links muito importantes para baixar e-Books gratuitamente). You can do the downloads of the documents about it!! Você pode fazer os downloads destes 2 documentos sobre este assunto!! @ List of so many links of this blog and the documents about it to do the downloads!! The Era of Modernity -> We live in a time when many companies, laboratories and industries from the most varied sectors and locations in the area of human health are increasingly improving the techniques and technologies in the diagnosis, prevention and treatment of various diseases, including those that are fatal and silent (go unnoticed by the person). However, with all this scientific and technological advance in this sector of society, unfortunately a large number of people around the world acquire numerous diseases of various degrees and stages, causing a large number of deaths daily. This contradiction and ambiguity occurs because there is an enormous difficulty in understanding the different biological and physiological mechanisms by which these diseases manifest themselves in people for a long time. The person’s genetics and their age are one of the related factors. with the morbidity and mortality of diseases, as it is known, in the over time, the human body accumulates many factors at the cell phone and there is also the natural wear and tear of it, as per example, the number of heartbeats in a certain interval of time, obviously. As many people say, the human body can be equivalent in a certain way to a machine that when passing of time, your organs (parts) will wear out due to the numerous internal and external factors, and thus life expectancy human tends to decay. Of course efficient drugs, medical devices for detection (diagnosis), prognosis and treatment of diseases, vaccines and therapeutic substances are great allies in the best quality of life and life expectancy person’s. An increasingly efficient personalized medicine with certainty is of great importance for human health too, taking into account the patient’s age and genetics, consequently. Obviously, this quest for perfection and high degree of efficiency in the development of new therapeutic substances, medicines, vaccines and medical devices involve many factors, among which the political, financial, research, professional training, construction or improvement of new pharmaceutical laboratories, companies and industries, among others. The breaking of paradigms and major challenges are extremely important for Science, without a shadow of a doubt. Professors, scientists, researchers and students must have great motivation and reference to obtain better results from their scientific work for society. This point has to be highlighted because unfortunately many health professionals are not well valued even in financial levels. Public authorities must be fully aware of this fact, obviously. Otherwise, the long-awaited scientific discoveries by society will take longer than “normal” to emerge. A matter of great importance to be discussed is the possibility of emergence of certain diseases (unknown diseases) in some country, several countries or even in all countries of the world. The complicated issue of this is the enormous difficulty of predict these diseases, as an example, it is worth remembering the emergence of COVID-19. The acquisition of very efficient technologies for this purpose, in case of detection of a possible possibility of the emergence of a disease unknown by the world scientific community, even more if this is fatal, it is of paramount importance for everyone. Beyond of the detection (diagnosis) of this type of disease, it is vital importance the existence of great technologies capable of preventing (preventing the disease from manifesting in the person) and even doing an excellent prognosis and treatment. The world scientific community needs to unite more and more in all its aspects for human interdependence to be very effective and thus the results obtained from research and projects they will be much better and satisfying for all mankind. With of course, the full and exact understanding of these types of diseases to avoid morbidity and mortality is very difficult, however, impossible. The human being has already conquered many things that were said by many people as impossible to be reached, like a lot of people know. The dissemination of knowledge and information from a reliable source is also of great value, of course, because as many already know, it is from there that many people learn about this information and knowledge conveyed by the Internet, radio and/or television and so on. progress of a country and even of the whole world, depending on the circumstances and the reach reached of diffusion (sharing) is reached more quickly. For a long time, research and academic projects have been fundamental for the technical-scientific and socioeconomic development of a country. That’s why more detailed and effective scientific research and projects are great allies to world progress. High-impact innovations are the ”soul” of world scientific progress. The era of Modernity has made life much easier for many people in several countries. The other side of the coin and balance of this era is that unfortunately there are still many people living in extreme poverty. presenting a terrible quality of life and life expectancy. In this sense, Science in all its aspects needs to achieve all sectors of society, including those countries where the of the most varied types of resources prevails. The authorities policies need to act much more in this field of society world, because many problems, including violence and hunger, arise due to the scarcity of financial resources in these locations, for example. Therefore, the most active and the protagonists in this era of Modernity in many countries urgently need to look, analyze and deeply reflect the big problems of several types in other countries without discrimination and without disrespect, because the Science needs to progress in all countries in a more fair. The right to a decent life is everyone’s right, no matter which country that the person is living in! Each country has made and makes its history! Unfortunately, there have been many injustices throughout history! The world needs to be more united, more and more! health and peace for all! @ Other relevant documents to do the downloads @ The diffusion of relevant information and knowledge is essential for a country progress always!! A difusão de relevantes informações e conhecimentos é sempre essencial para o progresso de um país!! @ Thank You!! Obrigado!! https://science1984.wordpress.com/2021/12/25/what-levels-of-influence-does-the-internet-have-on-peoples-lives-in-its-various-aspects-the-fact-is-that-many-situations-that-occurred-and-occur-daily-in-the-internet-environment-would-never-occur/ – What levels of influence does the Internet have on people’s lives in its various aspects? The fact is that many situations that occurred and occur daily in the Internet environment would never occur in these people’s lives, due to several interconnected factors, since this virtual world is very complex and dynamic and is very linked to imagination and assumptions, for example. Depending on the circumstance and other factors, a lot of information that one person typed for another, he would not speak or read what he typed. Obviously the Internet has its very good and important side in people’s lives in many aspects. However, it has its negative and dark side in several interconnected issues, coming to be dangerous in the lives of many people, unfortunately. The Internet has two sides of the coin, one positive and one negative. Furthermore, as many of you already know, there is a huge amount of money directly and/or indirectly related to the Internet, which is strongly linked to the economy of a certain place. As you already know, the Internet originated from the great ideas and thoughts of people and since then, a lot has changed and has been changing constantly, causing each time another significant increase in the number of Internet users in various parts of the world. There are several types of influences in different intensities that the Internet can cause in people’s lives, depending, for example, on the types of website content that people access and their frequency of access, as well as there are different means of communication, such as the writing, speaking, writing something, and others. Certainly, there are sites that are much better than others in various subjects, causing a different type of behavior and view of the Internet user on this site. As many say, there is a virtual world very different from the real world. Obviously, the different technologies developed by people have impacts of different degrees on people’s lives, bringing different types of consequences, for example, very constructive and very negative and dangerous. Another very obvious thing is that not always what happened and/or happens in the present in the virtual environment happened and/or will happen in a personal contact between 2 people in certain circumstances, for example. Therefore, it is necessary to be very careful when browsing the Internet, as there are a lot of sites of different types, some much better than others on the same topic, causing different degrees of influence on people’s minds and actions at different time intervals, depending a lot on the usage time of a certain website, for example. Much Health and Peace for You Always!! #probability #reader #time #pioneer #USA #pioneers #ideas #people #person #thoughts #thinking #memory #tempo #probabilities #website #videos #links #websites #video #photo #images #photos #names #name #DifferentInfluences #HumanMind #Feelings #intentions #goal #objectives #tests #work #Internet #HumanInterdependence #test #life #speak #sincerity #virtual #real #HumanMinds #actions #life #health #probabilities #virtuality #reality #countries #economy #world @ YouTubeVideo – @ Who invented the Internet? And Why? Quem inventou a internet? E por que? https://www.youtube.com/watch?v=21eFwbb48sE www.youtube.com & https://www.youtube.com/watch?v=KF6sLCeBj0s Como a internet funciona? – Glad You Asked T1 – How does the Internet work? https://science1984.wordpress.com/2022/12/14/66091/ – Probabilities -> More Interactions of Many Types -> Groups of Retired People on Social Networks – Email Lists of Retired People Who Worked in Excellent Research and Teaching Centers of the World @ Probabilidade(s) -> Maiores Interações de Muitos Tipos -> Grupos de Pessoas Aposentadas em Redes Sociais – Lista de Emails de Pessoas Aposentadas que Trabalharam em Excelentes Centros de Pesquisa e Ensino do Mundo @ ´´What happens in the digital world does not always happen in human life without the internet, obviously ´´. Rodrigo Nunes Cal @ ´´Nem sempre o que ocorre no mundo digital ocorre na vida humana sem a internet, obviamente´´. Rodrigo Nunes Cal https://science1984.wordpress.com/2023/01/08/100-cem-sem-no-sem-dna-cell-no-thinking-reading-pensamentos-chat-leituras-no-time-tempo-nos-tempos-times-cem-100-pensamentos-leituras-no-papel-paper-https-science1984-w/ -@ 100 – cem – sem – no (sem) DNA/Cell – no thinking/reading/pensamento(s)/chat/leitura(s) – No time/tempo – No(s) tempo(s)/time(s) – cem (100) pensamentos/leituras – no papel/paper https://science1984.wordpress.com/2022/07/30/internet-history-pionner-pionners-work-world/ https://science1984.wordpress.com/2022/12/07/goals-of-this-blog-e-mails-probabilities-reading-leituras-usa-authorities-team-times-tempos-memory-law-lei/ www.wikipedia.org www.usa.gov www.fbi.gov www.cia.gov www.forbes.com www.wordpress.com www.stripe.com www.nature.com www.nejm.com www.nobelprize.org www.ucla.edu www.harvard.edu www.mit.edu www.stanford.edu https://www.cam.ac.uk/ https://www.ox.ac.uk/ https://ki.se/ www.bbc.com www.bloomberg.com www.cnn.com https://www.wsj.com/ https://www.uspto.gov/ www.nih.gov www.cdc.gov www.fda.gov www.pfizer.com www.gmail.com www.yahoo.com www.google.com www.youtube.com www.twitter.com www.facebook.com www.facebook.com/scientificblog www.linkedin.com www.instagram.com www.yale.edu www.cornell.edu www.duke.edu www.famerp.br www.uftm.edu.br www.ita.br www.embraer.com www.gov.br/pt-br www.unesp.br https://www5.usp.br/ https://www.unifesp.br/ https://ufmg.br/ www.ufrgs.br https://portal.uel.br/home/ https://www5.pucsp.br/paginainicial/www.ufscar.br https://www.gov.br/pf/pt-br https://www.stj.jus.br/sites/portalp/Inicio https://pt.semrush.com/blog/top-100-sites-mais-visitados/ https://www.whitehouse.gov/contact/ https://science1984.wordpress.com/2022/12/14/66104/ > ´´Obstacles are opportunities in disguise.´´ Deepak Chopra https://science1984.wordpress.com/2022/12/14/66086/ – Time/Tempo – Action/Ação – Influence/Influência – Internet/Person/People/Pessoa(s) – Probabilities/Possibilidades – Laws/Leis – Money/Dinheiro/Price/Preço/Justice/Justiça – Intentions/Intenções – Facts/Fato(s) – Life/Vida(s)/Lives/Health/Saúde – World History – Tempo de Vida/Lifetime https://science1984.wordpress.com/2022/12/13/graphics-comparisons-my-dissertation-relevant-reference-human-and-animal-researches-ages-age-person-people-analysis-of-influence-of-many-biological-factors-related-to-relation-among-health/ -Graphic Comparisons – Reference – Human and Animal Researches – Ages/Age – Person/People – Study of factors related to relation among health, time, diseases and death. https://science1984.wordpress.com/2022/12/13/cell-dna-time-life-lives/ – Cell/DNA/People – Person/Age/Ages – DNA/RNA – Human Life/Health – Times – Human Lives https://science1984.wordpress.com/2022/12/13/there-was-no-statistically-significant-difference-and-now-nao-houve-diferenca-estatistica-significativa-e-agora-editorial-20-march-2019-nature-567/ – ´´There was no statistically significant difference. And now? ´´Não houve diferença estatística significativa. E agora?´´ @ EDITORIAL 20 MARCH 2019 – Nature 567, 283 (2019) -> ´´It’s time to talk about ditching statistical significance – Looking beyond a much used and abused measure would make science harder, but better.´´ É hora de falar sobre a redução da significância estatística – Olhar além de uma medida muito usada e abusada tornaria a ciência mais difícil, mas melhor. ´´If researchers do discard statistical significance, what should they do instead? They can start by educating themselves about statistical misconceptions. Most important will be the courage to consider uncertainty from multiple angles in every study. When working out which methods to use, researchers should also focus as much as possible on actual problems. People who will duel to the death over abstract theories on the best way to use statistics often agree on results when they are presented with concrete scenarios.´´ & WHAT DOES STATISTICALLY SIGNIFICANT MEAN? by Jeff Sauro, PhD | October 21, 2014 @ Wikipedia Links about Time, Ageing, Age and Nobel Foundation & Nobel Prize Website – www.nobelprize.org @ OTHER VERY IMPORTANT INFORMATION @ https://www.nature.com/articles/d41586-019-00857-9 @ https://www.nature.com/articles/d41586-019-00874-8 https://www.tandfonline.com/doi/full/10.1080/00031305.2019.1583913 https://science1984.wordpress.com/2022/12/09/as-many-people-know-not-every-theory-can-be-fully-or-partially-applied-in-practice-always-keep-in-mind-wanting-to-improve-the-society-in-some-way-whatever-it-may-be-rodrigo-nunes-cal/ – ´´As many people know, not every theory can be fully or partially applied in practice. Always keep in mind wanting to improve the society in some way, whatever it may be´´. Rodrigo Nunes Cal @ ´´Como muitos já sabem, nem toda teoria consegue ser totalmente ou parcialmente aplicada na prática. Tenha sempre em mente em querer melhorar a sociedade de alguma forma, seja ela qual for´´. Rodrigo Nunes Cal @ https://www.genome.gov/ www.usa.gov www.nobelprize.org www.forbes.com www.fbi.gov @ Internet #image #foto(s) #photo #images #age #idade(s) #probabilities #probabilidades #person #feelings #sentimentos #thinking #thoughts #actions #pensamento(s) #ações #time #future #tempo #present #presente #futuro #past #passado #people #visão #vision #país #country #cidade #city #memory #memória #mente #leitura #reading #life #vida #saúde #mind #health #pessoas #pessoa #friends #friend #family #world Internet #image #foto(s) #photo #images #age #idade(s) #probabilities #probabilidades #person #feelings #sentimentos #thinking #thoughts #actions #pensamento(s) #ações #time #future #tempo #present #presente #futuro #past #passado #people #visão #vision #país #country #cidade #city #memory #memória #mente #leitura #reading #life #vida #saúde #mind #health #pessoas #pessoa #friends #friend #family #world #ChangeOfOpinion #MudançaDeOpinião #LifeFacts #FatosDaVida @ ´´The external temperature of the planet affects to the internal temperature of the planet, the difference between the internal and external temperature produce the magnetic field force. If this difference of temperature reduces, the magnetic field decreases reducing the rotational velocity of the planet and also reduce the inclination. If the inclination reduces, also the sun-rays with higher energy can enter in the planet producing the increase of the external temperature. And it happens exponentially, and can be applied to all bodies also to the Sun. I applied it to the Sun, venus, Earth, Mars Jupiter and Saturn with satisfactory results´´. Joan Carles Testagorda Garcia joancarles@hotmail.es Astrophysisc – Quantum Physics, Mathematics. @ https://www.nytimes.com/international/section/opinion @ https://www.nytimes.com/international/section/opinion www.cnn.com www.bbc.com www.usa.gov #opinion #opinião #OpinionChange #MudançaDeOpinião #assunto #subject #person #pessoas #people #person #AllProbabilities #TodasProbabilidades #Internet #Vision #visão #communication #comunicação https://science1984.wordpress.com/2022/11/28/i-used-to-believe-that-conversation-was-the-key-to-everything-but-actually-understanding-it-is-because-you-can-say-everything-that-bothers-you-but-if-the-other-doesnt-understand-it/ ´´I used to believe that conversation was the key to everything. But today comprehension it is. Because you can say everything that bothers you, but if the other doesn't understand, it's over.´´ https://science1984.wordpress.com/2022/11/14/animal-and-human-testing-testes-em-animais-e-em-humanos/ – Animal and human testing @ Phases of clinical research – Clinical research is conducted on drug candidates, vaccine candidates, new medical devices, and new diagnostic assays @ Highly Cited Researchers (h>100) according to their Google Scholar Citations public profiles – 15th Edition – second week of March 2022 @ Sharing interesting and important informations is a very important act because it helps in the diffusion of them in the world. Of course, it has to be true and not fake news. https://science1984.wordpress.com/2022/10/09/not-always-what-we-think-refers-to-what-we-are-doing-and-or-what-we-want-to-do-in-the-future-ask-yourself-mentally-or-not-about-anything-whatever-the-subject-with-your-very-sincere-a/ -> ´´Not always what we think refers to what we are doing and/or what we want to do”. Rodrigo Nunes Cal @ ´´Nem sempre o que pensamos refere-se àquilo que estamos fazendo e/ou que queremos fazer´´. Rodrigo Nunes Cal https://science1984.wordpress.com/2022/07/30/the-human-expectancy-of-life-needs-to-increase-so-much-faster-more-and-more-with-very-efficient-researches-and-projects-resulting-in-new-vaccines-therapeutical-substances-medical-devices-and-drugs/ – ´´The human expectancy of life in the world needs to increase so much faster more and more with very efficient scientific researches and projects resulting in new ideas, references, vaccines, therapeutical substances, medical devices, drugs, prevention, diagnostic, prognostic and treatment methods, for example´´. Rodrigo Nunes Cal @ https://science1984.wordpress.com/2018/11/15/hi-rodrigo-we-are-the-globo-tv-international-relationship-center-congratulations-to-you-for-all-the-works-you-are-doing-with-your-studies-its-very-good-to-know-about-it-thanks-for-sh/ @ www.google.com www.time.com www.gmail.com www.yahoo.com www.usa.gov www.nih.gov www.wikipedia.org www.wordpress.com www.genome.gov www.nobelprize.org www.harvard.edu www.mit.edu www.columbia.edu www.nasa.gov www.ucla.edu www.stanford.edu www.princeton.edu www.yale.edu www.duke.edu www.cornell.edu www.nyu.edu www.caltech.edu www.nyt.com www.wsj.com www.reuters.com www.youtube.com www.bloomberg.com www.ft.com www.nasdaq.com www.forbes.com www.exame.com www.famerp.br www.uftm.edu.br www.ita.br www.cia.gov www.fbi.gov www.facebook.com www.instagram.com www.linkedin.com www.twitter.com www.amazon.com www.spacex.com www.tesla.com www.microsoft.com www.alibaba.com www.apple.com www.stripe.com www.wellsfargo.com www.worldbank.org https://science1984.wordpress.com/2022/08/10/you-have-a-very-impressive-career-ahead-of-you-very-good-worki-agree-with-you-100-we-dont-live-long-enough-ill-be-60-next-month-and-i-have-at-least-100-years-of/ – ´´You have a very impressive career ahead of you. Very good work´´I agree with you 100%. We don´t live long enough. I´ll be 60 next month and I have at least 100 years of work I need to do.´´ no joke… There´s always too much todo and I´ve lost so many good friends over the years. good people…´´ -> Messgaes for me from my Facebook Friend Alan House ´´I am a Software Engineer and have had my own business since 1982´´ https://science1984.wordpress.com/2022/08/06/facebook-messages-for-me-from-american-college-of-medical-genetics-and-genomics-acmg-after-ive-sent-it-messages-about-this-blog-thank-you-good-luck-in-your-work-thanks-for-reach/ – Facebook messages for me from American College of Medical Genetics and Genomics (ACMG) after I´ve sent it messages about this blog: ´´Thank you. Good luck in your work! Thanks for reaching out and we wish you all the best with your blog.´´ – American College of Medical Genetics and Genomics -> Telephone: 301-718-9603 Fax: 301-718-9604 E-mail: acmg@acmg.net 7101 Wisconsin Avenue – Suite 1101 Bethesda, MD 20814 @ www.google.com www.apple.com www.amazon.com www.forbes.com www.nih.gov www.usa.gov www.gmail.com www.yahoo.com www.youtube.com www.harvard.edu www.mit.edu www.nasa.gov www.princeton.edu www.columbia.edu www.caltech.edu www.yale.edu www.cornell.edu www.duke.edu www.pfizer.com www.linkedin.com www.microsoft.com www.twitter.com www.nobelprize.org www.wordpress.com www.stripe.com www.facebook.com www.facebook.com/scientificblog www.cnn.com www.bbc.com www.fbi.gov www.cdc.gov www.fda.gov www.cia.gov www.alibaba.com www.spacex.com www.tesla.com www.timeshighereducation.com www.wikipedia.org www.exame.com www.ita.br www.famerp.br www.uftm.edu.br www.embraer.com www.instagram.com www.whatsapp.com www.ox.ac.uk www.ki.se www.u-tokyo.ac.jp www.wellsfargo.com www.bloomberg.com www.nasdaq.com www.washingtonpost.com www.ft.com www.ucla.edu https://science1984.wordpress.com/2022/08/14/61244/ Sempre serei do bem. I´ll always be a good person. @ World History – História Mundial – 100 Genes – No Gene/Sem gene – Numbers/DNA -Read/Listen/Speak/Type/Write – Country https://science1984.wordpress.com/2022/08/24/61708/ – DNA/Cell – 100-Pensamento(s) – Thinking – Read @%?https://www.goodreads.com/author/quotes/7221234.Elon_Musk https://science1984.wordpress.com/2022/08/27/the-first-step-is-to-establish-that-something-is-possible-then-probability-will-occur-elon-musk-o-primeiro-passo-e-estabelecer-que-algo-e-possivel-entao-a-probabilidade/ – “The first step is to establish that something is possible then probability will occur.” Elon Musk @ “O primeiro passo é estabelecer que algo é possível, então a probabilidade ocorrerá.” Elon Musk & Probability – Thinking – Facts – Speak – Listen – Write – Type – Actions – Think – Reading – Life – Health – Mind – Person – People – Minds – Same Times – Same Time – Different Probabilities – Real Fact – Intentions – Objectives – Feelings – Time – Question – Deep Self-Reflection – Affirmation – Learning – Human Errors – Information – Human Rights – Human Evolution – World Progress – Human Longevity – Pionners – Work – Prices – Money – Laws – Cities – Countries – Motivation – Logic Analysis Of The Different Times – Television – Internet – Radio – Cell – Live – Different Lives – DNA – Subject – Reflections – Imaginations – Future – Probabilities – Different Times – Different Thoughts – Same Thoughts – Mind Inductions – Telepathy – Very High Coincidences – Mind Reading – Human Interdependence – Superations – Negatives Facts – Positive Facts – World History – Internet History – Human Brains – Human Brain – Respect – Human Qualities – Internet Pionner – Internet Pionners – Computer Pionner – Cell Phone Pionner – Medical Devices Pionners – Books – eBooks – Free eBooks – Different Intensities of Determined Subjects – World Contributions – Names – Name – Supositions – Nome – Nomes – Internet – Time – Times – Thinking – Pensamento(s) – Thoughts – Progress – New Ideas – Novas Ideias – Novos Projetos – New Projects – New Research – Novas Pesquisas – Citations – References – Knowledge – Different Countries – Different and Same Departments – Information – People – Person – Human Longevity – New Scientific Discoveries – Scientific Community – USA Authorities – Internet Pionner – First Idea – Work – Live – Life – Future Generations – Different Jobs – Investigations – Personal History – Team – Govern – Police – Researches – Projects – Ideas – Imagination – Different Jobs – Very High Importance of the Human life – Very High Importance of Human Lives – Messages – Meeting – Meetings – Scientific Events – Relevant Researches – Excellent References – Message – Technologies – Graphics – Data – Origin – Notebooks – Computers – Authorities – Logic – Future Facts – Place – Level of Importance – RANKING – Internet Content – Website Content – Websites – Different Communication – Blog Content – Links – Videos – Photos – Images – Analysis – Different Countries – Understanding – Friends – Friend – Future Times – Past Times – Present Time – Normal Facts of Human Life https://science1984.wordpress.com/2022/10/16/the-17-equations-that-changed-the-world-andy-kiersz-mar-31-2016-601-pm-https-www-businessinsider-com-equations-that-changed-the-world-2016-3-www-google-com-www-forbes-com-www-gmail-com-www/ - The 17 equations that changed the world - Andy Kiersz - Mar 31, 2016, 6:01 PM https://www.businessinsider.com/equations-that-changed-the-world-2016-3 www.google.com www.forbes.com www.gmail.com www.yahoo.com www.usa.gov www.nih.gov www.wikipedia.org www.nobelprize.org www.wordpress.com www.youtube.com www.facebook.com www.twitter.com www.linkedin.com www.instagram.com www.amazon.com www.tesla.com www.nasa.gov www.harvard.edu www.mit.edu www.stanford.edu www.ucla.edu www.caltech.edu www.yale.edu www.columbia.edu www.cnn.com www.bbc.com www.cornell.edu www.princeton.edu www.alibaba.com www.facebook.com/scientificblog www.fbi.gov www.cia.gov www.fda.gov www.cdc.gov www.pfizer.com www.apple.com www.microsoft.com www.duke.edu www.famerp.br www.ita.br www.uftm.edu.br www.embraer.com www.pubmed.ncbi.nlm.nih.gov www.nature.com www.nejm.com www.gov.br https://lattes.cnpq.br https://science1984.wordpress.com/2022/09/14/the-world-needs-to-improve-so-much-faster-with-very-efficient-ideas-researches-and-projects-resulting-in-new-drugs-therapeutic-substances-medical-devices-vaccines-and-others-unfortunately-there/ – The world needs to improve so much faster with very efficient researches and projects, resulting in new drugs, medical devices, vaccines, therapeutic substances and others things. Unfortunately there are fatal diseases and/or unknown diseases https://science1984.wordpress.com/2022/09/07/human-mind-mente-vidas-life-lives/ EU/I – Pensamento(s) da(s) Pessoa(s) – Thinking of the Person/People www.gmail.com www.yahoo.com https://science1984.wordpress.com/2022/09/29/but-science-is-the-collection-of-natures-answers-the-humanities-the-collection-of-mens-thoughts-sir-gavin-de-beer-https-science1984-wordpress-com-2022-09-29-s/ ´´But science is the collection of nature’s answers; the humanities the collection of men’s thoughts.´´ — Sir Gavin de Beer https://science1984.wordpress.com/2022/09/29/science-quotes-answer-quotes/ https://science1984.wordpress.com/2022/08/06/acmg-for-me-by-facebook-thank-you-good-luck-in-your-work-thanks-for-reaching-out-and-we-wish-you-all-the-best-with-your-blog-genetics-cell-dna-researches-ideas/ ACMG for me by Facebook -> ´´Thank you. Good luck in your work! Thanks for reaching out and we wish you all the best with your blog.´´ @ #Genetics #Cell #DNA #Research #Ideas #Projects #Efficiency #HumanLongevity #Age #Ages #Time #Times #Life #Lives #Work #Blog #Internet #Reading #Feelings #Health #Mind #Minds #Contributions #People #Person #Links #Videos #Images #Data #Websites #SocialNetworks #Speak #thinking #Thoughts #Write #Type #Pioneers #Pioneer #Communication #Progress #Country #Countries #Cities #City #Economy #Price #Information #Money #Knowledge #Prices #Probability #Click #Clicks #Text #Texts #Intentions #Goal #Objectives #ScientificCommunity #WorldInnovation #Graphics #Dissertations #Monographs #References #Citations #ImpactFactors #Journals #TrueFacts #Science #ScientificDiscoveries #Hope #FutureGenerations #Evolution #HardWork #Analysis #Investigation #Ranking https://science1984.wordpress.com/2021/11/29/message-that-my-facebook-friend-roger-weiss-who-works-at-nasa-sent-for-me-very-best-success-with-your-blog-and-research-some-of-positive-feedbacks-i-received-about-this-blog/ – Message that Roger Weiss who works at NASA sent for me by Facebook: ´´Very best success with your blog and research´´ @ Some of positive feedbacks I´ve received about this blog by LinkedIn and Facebook @ Statistics about this blog in this day (12/01/2021) and others periods & YouTube Video (George Church on reversing aging | ApplySci @ Harvard) @ Relevant Links, YouTube Videos (Internet Pioneers), Websites, Social Networks and Images @& ´´Nothing is better than reading and gaining more and more knowledge.” — Stephen Hawking https://science1984.wordpress.com/2020/03/07/fome-e-o-fator-que-leva-a-celula-tumoral-a-migrar-pelo-corpo-diz-pesquisador-hunger-is-the-factor-that-causes-the-tumor-cell-to-migrate-through-the-body-says-researcher-genome-sequencing-key-to/ – Twitter message for me from @IEEEorg about this blog: ´´Thanks for your hard work!´´ @ Nature 567, 283 (2019) doi: 10.1038/d41586-019-00874-8 – EDITORIAL 20 MARCH 2019 – ´´It’s time to talk about ditching statistical significance Looking beyond a much used and abused measure would make science harder, but better. If researchers do discard statistical significance, what should they do instead? They can start by educating themselves about statistical misconceptions. Most important will be the courage to consider uncertainty from multiple angles in every study. Logic, background knowledge and experimental design should be considered alongside P values and similar metrics to reach a conclusion and decide on its certainty. Researchers should seek to analyse data in multiple ways to see whether different analyses converge on the same answer.´´ @ Fome é o fator que leva a célula tumoral a migrar pelo corpo, diz pesquisador – Hunger is the factor that causes the tumor cell to migrate through the body, says researcher #@ Genome Sequencing: Key to solving India’s rare diseases problem & CRISPR treatment inserted directly into the body for first time – Experiment tests a gene-editing therapy for a hereditary blindness disorder @ Coronavirus disease 2019 (COVID-19): News and Information – Massachusetts General Hospital & ´´Não houve diferença estatística significativa. E agora?´´ @ ´´WHAT DOES STATISTICALLY SIGNIFICANT MEAN?´´ by Jeff Sauro, PhD | October 21, 2014 @ OTHER VERY IMPORTANT INFORMATION OF THE WORLD LIKE WEBSITES, LINKS AND IMAGES & The graphics I did about ´´my´´ dissertation that can be great reference for many types of future researches. Os gráficos que fiz sobre ´´minha´´ dissertação que podem ser ótima referência para futuras pesquisas de vários tipos @ https://inano.au.dk/about/research-groups/gothelf-lab https://inano.au.dk/open-positions/open-phd-positions https://science1984.wordpress.com/2020/12/31/twitter-messages-for-me-from-my-twitter-follower-joe-w-joewer32145-who-is-mechanical-engineer-at-nasa-nasa-about-my-research-i-told-my-colleague-about-your-researchhe-thinks/ – Twitter messages for me from my Twitter follower Joe W. @joewer32145 who is mechanical engineer at NASA @nasa about my research ´´I told my colleague about your research´´He thinks you will be relevant in one of our research centres ´´It all depends on you´´You are a bright young man´´Okay´´I will communicate with you tomorrow noon´´ @ Other Twitter message for me about the message I sent to the doctor Rasu Shrestha @RasuShrestha ´´Thanks for sharing the link to your blog Rodrigo! All the best to you in 2021.´´ @ My Facebook Page, Curriculum Lattes and YouTube Channel – My Social Networks and E-mails Accounts & Images, links, other Twitter messages and Great websites @ Many people liked the comments I did about Tweets of other people. Images about it are available in this Blog @ Image and link about the most powerful computers in the World – Top500: Japan’s Fugaku Still the World’s Fastest Supercomputer @ Images about my new Twitter Followers @ ´´The wise man never says everything he thinks, but he always thinks everything he says´´. Aristotle @ ´´O sábio nunca diz tudo o que pensa, mas pensa sempre tudo o que diz.´´ Aristóteles & Instagram message for me about this blog from Karolinska Institutet Students https://www.instagram.com/kistudents/ ´´Thank you for your message and for sharing your blog with us. As an account, we unfortunately cannot share this external content with our audience, but we appreciate that you reached out, and we wish you the best of luck! Kind wishes, Gerda 😊 Same to you! Gerda´´. Instagram message I sento to Karolinska Institutet Students: If possible, visit and share my https://science1984.wordpress.com because this blog content is very good with very quality and importance to the world. I do not earn any money from it. Many people worldwide have visited it and liked it such as renowned professors, scientists and/or researchers around the world!! The people need to live better and longer, more and more!! So, this blog sharing is fundamental to the world progress always!! Inform to your friends to do the same and so on!! Let´s make a very big positive chain of dissemination of it!! Thanks for your attention!! Sorry for anything. #probability #time #reader #countries #city #cities #country Best regards !! Thank You !!! Best wishes always for you, your friends and family!! @ ´´The difficulty in concentrating can have both internal causes, such as a mental disorder, and external causes, such as the work environment or cell phone. ” @ ´´A dificuldade para se concentrar pode ter tanto causas internas, como um transtorno da mente, quanto causas externas, como ambiente de trabalho ou celular. ´´- Twitter messages for me from my Twitter follower Joe W. @joewer32145 who is mechanical engineer at NASA @nasa about my research ´´I told my colleague about your research´´He thinks you will be relevant in one of our research centres ´´It all depends on you´´You are a bright young man´´Okay´´I will communicate with you tomorrow noon´´ @ Other Twitter message for me about the message I sent to the doctor Rasu Shrestha @RasuShrestha ´´Thanks for sharing the link to your blog Rodrigo! All the best to you in 2021.´´ @ My Facebook Page, Curriculum Lattes and YouTube Channel – My Social Networks and E-mails Accounts & Images, links, other Twitter messages and Great websites @ Many people liked the comments I did about Tweets of other people. Images about it are available in this Blog @ Image and link about the most powerful computers in the World – Top500: Japan’s Fugaku Still the World’s Fastest Supercomputer @ Images about my new Twitter Followers @ https://science1984.wordpress.com/2019/04/06/facebook-messages-for-me-dear-mr-rodrigo-cal-thank-you-very-much-for-your-message-and-the-information-about-your-research-it-is-very-important-information-in-xx/ – Facebook messages for me from Oxford Union: ´´Dear Mr. Rodrigo Cal!´´ ´´Thank you very much for your message and the information about your research. It is very important information in XXI century. The Academic Union of Oxford has a lot of projects in all kinds of the scientific areas. This year our team is preparing projects for Medical Universities all over the world. Please follow our news to know the most actual conferences and events in scientific and educational area! Best regards! Julia May, Manager of the Academic Union, Oxford´´ @ Some websites about animal researches &@ ´´The Academic Union is an international academic association of over 200 University Chancellors, Scientists and Researchers. The committees and the Union as a whole pursue the ideals of Academic Excellence, Innovation and Accessibility and are committed to disseminating these core values through its worldwide community.The primary aim of the Union is to consolidate the efforts of scientists, educators, industrialists, and manufacturers on science, education and business integration and development in the XXIst century.´´ @ https://science1984.wordpress.com/2018/11/15/hi-rodrigo-we-are-the-globo-tv-international-relationship-center-congratulations-to-you-for-all-the-works-you-are-doing-with-your-studies-its-very-good-to-know-about-it-thanks-for-sh/ – ´´Hi Rodrigo. We are the Globo TV International Relationship Center. Congratulations to you for all the works you are doing with your studies. It’s very good to know about it. Thanks for sharing your messages with us. Before we go ahead with our conversation, could you please answer some questions? In which country do you live and by which provider do you subscribe our TV channel? We await for your response.´´ #time www.google.com www.usa.gov www.forbes.com www.exame.com www.nobelprize.org www.harvard.edu www.mit.edu www.stanford.edu www.facebook.com www.twitter.com www.youtube.com www.gmail.com www.yahoo.com www.instagram.com www.linkedin.com #history #country #countries #communication #analysis #investigations #intentions #mind #feelings #health #life #age #ages #name #cities #city #person #blog #system #people #work #lives #contribuitions #importance #respect #subject #subjects #goal #objectives #reading #speak #type #listen #DifferentThings #DifferentTechnologies #DifferentInluences #FutureGenerations #longevity @ https://globointernacional.globo.com/Paginas/sinais.aspx https://falecomaredeglobo.globo.com/- ´´Hi Rodrigo. We are the Globo TV International Relationship Center. Congratulations to you for all the works you are doing with your studies. It’s very good to know about it. Thanks for sharing your messages with us. Before we go ahead with our conversation, could you please answer some questions? In which country do you live and by which provider do you subscribe our TV channel? We await for your response.´´ #time www.google.com www.usa.gov www.forbes.com www.exame.com www.nobelprize.org www.harvard.edu www.mit.edu www.stanford.edu www.facebook.com www.twitter.com www.youtube.com www.gmail.com www.yahoo.com www.instagram.com www.linkedin.com #history #country #countries #communication #analysis #investigations #intentions #mind #feelings #health #life #age #ages #name #cities #city #person #blog #system #people #work #lives #contribuitions #importance #respect #subject #subjects #goal #objectives #reading #speak #type #listen #DifferentThings #DifferentTechnologies #DifferentInluences #FutureGenerations #longevity @ https://globointernacional.globo.com/Paginas/sinais.aspx ´´No passado, eu achava que a conversa era a chave de tudo. Mas na verdade, a compreensão que é, pois você pode falar tudo o que te incomoda e se o outro não entender, já era.´´ https://science1984.wordpress.com/2022/11/28/antigamente-eu-acreditava-que-a-conversa-era-a-chave-de-tudo-mas-na-verdade-a-compreensao-que-e-porque-voce-pode-falar-tudo-o-que-te-incomoda-porem-se-o-outro-nao-entender-ja-era/ https://science1984.wordpress.com/2022/04/24/different-types-of-internet-connections-submarine-cable-map-https-www-submarinecablemap-com-https-www2-telegeography-com/ Different Types of Internet Connections @ Submarine Cable Map – https://www.submarinecablemap.com/ @ https://www2.telegeography.com/ https://science1984.wordpress.com/2022/01/28/genetic-engineering-images-links-websites-and-youtube-videos-01-28-2022/ – Genetic Engineering -> Images, Links, Websites and YouTube Videos (01/28/2022) https://science1984.wordpress.com/2022/01/31/graphics-bigchallenge-feedbacks-database-scientificcommunity-person-people-age-ages-genetics-time-of-life-of-each-person-in-the-world-reading-present-past-future-internetmessage/ – Graphics @# ´´My´´Dissertation &# ´´My´´Monograph @ # BigChallenge – World #Feedbacks #DataBase # ScientificCommunity #PERSON #PEOPLE #AGE #AGES #GENETICS – DATA & Thinking – Time of Life of Each Person in the World – Diseases – Fatal diseases – Reflections – Unknown Diseases – Rare Diseases – Times @ Indetermined Time – Physical Immortality @ #reading #present #past #future # InternetMessages #statistics #facts #research #genetics #age # HumanExpectancyOfLife #ages #person #AGE #PEOPLE #Genetics #HumanLongevity # WorldHistory # InternetHistory #Energies #time #life #I #EU #Interpretations # reading #Computer #people researches #professors #scientists #students #doctors #researchers # ExcellentResearchCenters #Countries #articles #efficiency #projects #tests #laboratories #work #intentions #ScientificDiscoveries #PharmaceuticalIndustries #reference #Biostatistics #biochemistry #physiology #MolecularBiology #DNA #Read #Cells #DifferentTimes #LIFE #lives #contribuitions #society #FutureGenerations #HEALTH ##TIME #HumanCell #DifferentTechnologies #GeneticEngineering #Science #Technologies #Innovations #drugs #vaccines #InternetImages #TherapeuticalSubstances #ideas #world #WorldInnovation www.science1984.com www.wordpress.com www.usa.gov www.nobelprize.org https://science1984.wordpress.com/2022/01/23/52592/ – Graphics of ´´my´´ dissertation and my monograph # WorldInnovation #Time # Feedbacks #Reading @ Others Data https://science1984.wordpress.com/2019/12/25/29845/ – What Happens in an Internet Minute in 2019? @#probability #probabilidade #reading #chat #leitura #tempo #respeito #world #word #name #nome #click #assunto #issue #content #conteúdo #idade #age #respect #investigations #investigações #pesquisa #researches #análise #equipe #team #analysis #ranking #sponsors #marketing #money #time #people #pessoas #pessoa #emails #e-mail #website #site #links #pergunta #question #afirmation #afirmação #leitor #reader #melhoria #improvement #videos #photos #images #MentalHealth #SaúdeMental #Health #Saúde #longevity #feelings #sentimentos #memory #memória @ LinkedIn message for me from my LinkedIn contact Professor Dr. Bankole Johnson, Executive Chairman of the Global Institutes on Addiction, and Founder and Chief Medical Officer of Adial Pharmaceuticals. He was Chair of the Department of Psychiatry, University of Maryland School of Medicine @ My LinkedIn Contacts https://science1984.wordpress.com/2019/12/15/29333/ – Memory/Memória(s) – I/EU @ Videos/Chat/Website/Brazil/Site(s)/HighPopularity/GrandePopularidade – Probability/Read my Messages in my Email Accounts about this Chat – USA/Brazil Authorities – True Facts about this Chat – Probabilities/Possibilidades – Intentions/Intenções – Leitura das minhas mensagens sobre este chat nas minhas contas de emails – Patrocinadores/Sponsors – Influence of this Chat in the Human Mind – High necessity of improvement in this chat – Grandes Iniciativas – Great Initiatives – Alta necessidade de melhorias neste chat – Saúde mental das pessoas – Mental health of people – Time – Tempo(s) – Times – País – Country – Cidade(s) – City – Cities – Economy – Economia – Saúde Pública – Public Health – Doença/Disease – Sentimentos/Feelings – Ações/Actions @ This Blog Contributions to the World Society and World Progress Now and Always # InternetHistory/Pioneers/ReconhecimentoDoTrabalho/WorkRecognition – Vida(s) – Lives – Life – Vida – Internet – Trabalho – Teams – Equipes – Futuras Gerações – Future Generations – Human Longevity – Longevidade Humana &# Artificially cloaked viral nanovaccine for cancer immunotherapy -> Nature Communications volume 10, Article number: 5747 (2019) @ 3 MILLION BITCOIN IN 2 SECONDS: GOOGLE QUANTUM COMPUTER @ VACCINES RESEARCH – DRUG DEVELOPMENT – DISEASE TESTING – VACCINE CANDIDATES – POTENTIAL POST-INFECTION THERAPIES – EFFECTIVE VACCINE – CLINICAL RESEARCH – POTENTIAL THERAPEUTICS DRUGS – INDUSTRY RESEARCHERS https://science1984.wordpress.com/2019/08/24/mri-scans-improve-prostate-cancer-detection-using-mri-scans-to-target-biopsies-is-more-effective-at-detecting-prostate-cancers-that-are-likely-to-need-treatment-than-standard-ultrasound-guided-biopsi/ – MRI scans improve prostate cancer detection. Using MRI scans to target biopsies is more effective at detecting prostate cancers that are likely to need treatment than standard ultrasound guided biopsies alone, according to research published in JAMA Network Open. @ Most important 21st century innovation | Arun Majumdar | TEDxStanford & Learn data science for beginners (How to learn data science for free)? @ Top 10 Technologies To Learn In 2020 | Trending Technologies In 2020 | Top IT Technologies | Edureka @ Understanding DevOps @ Prostate Cancer: Evolving Paradigms: From Biopsy to Treatment @ Prostate Cancer 101 | #UCLAMDChat Chat @ What Everyone Should Know About Prostate Cancer @ Very Important Videos https://science1984.wordpress.com/2019/06/17/ja-que-rola-a-zueira-ha-muito-tempo-em-um-site-ok-tudo-bem-since-it-has-been-mess-a-long-time-at-a-website-ok-it-is-all-right-policiafederal-fbi-email-leitura-reading-internet-emails-wor/ - Já que rola a zueira há muito tempo em um site, ok. Tudo bem. Since it has been mess a long time at a website, ok. It is all right. Por Um Mundo Melhor Sempre! For A Better World Forever ! @ Turning Drug Delivery Inside Out How spherical nucleic acid (SNA) technology may solve the challenge of delivering oligonucleotides into cells @ ´´Chaos theory is a branch of mathematics focusing on the study of chaos—states of dynamical systems whose apparently-random states of disorder and irregularities are often governed by deterministic laws that are highly sensitive to initial conditions.[1][2]´´ @ VERY IMPORTANT SOCIAL NETWORKS, WEBSITES, LINKS AND IMAGES OF THE WORLD #time #intentions #blog #country #countries #system #technologies #DifferentTechnologies #reality #DifferentPerceptions #mind #actions #influences #person #people #history #improvement #reading #respect #age #ages #researches #innovation #reference #affirmation #question #discussion #Science #energy #longevity #feelings #life #health https://science1984.wordpress.com/2019/01/22/chinese-facial-recognition-will-take-over-the-world-in-2019-michael-k-spencer-medium-member-since-apr-2018-blockchain-mark-consultant-tech-futurist-prolific-writer-wechat-mikekevinspencer-https/ Chinese Facial Recognition Will Take over the World in 2019 – Michael K. Spencer Medium member since Apr 2018 Blockchain Mark Consultant, tech Futurist, prolific writer. WeChat: mikekevinspencer https://medium.com/futuresin/chinese-facial-recognition-will-take-over-the-world-in-2019-520754a7f966?fbclid=IwAR0nUeBlb3hvxdKL4tjNM2A2oXVrs260bjhhx9AM3p6lfitu6IVlJ_NjFw4 https://science1984.wordpress.com/2018/12/20/15362/ #emails #incerteza #uncertain #Time #reading #leitura #imagination #video #chat #website #respect #melhorias #assunto #issue #improve #fact #patrocinadores #sponsors #Tempo #collaborations #USA #estratégiasmaisfáceis #mynotebook #firststrategy #MyBlogGoal #easierstrategies #Print #intelligence #VeryBigChallenge #internet https://science1984.wordpress.com/2018/10/20/our-administrator-chats-with-astronaut-nick-hague-osiris-rex-brakes-for-a-certain-asteroid-and-what-landing-site-is-right-for-our-next-mission-to-mars-a-few-of-the-sto/ ´´Our administrator chats with astronaut Nick Hague, OSIRIS-REx “brakes” for a certain asteroid, and what landing site is right for our next mission to Mars? A few of the stories to tell you about – This Week at NASA!´´ #TIME https://www.facebook.com/ThisWeekAtNASA/ https://www.nasa.gov/ https://science1984.wordpress.com/2018/08/15/us-court-issues-injunction-against-open-access-publisher-omics/ US Court Issues Injunction Against Open-Access Publisher OMICS – https://www.the-scientist.com/the-nutshell/us-court-issues-injunction-against-open-access-publisher-omics-30581 & Researchers Develop Wearable Microfluidic Cytometer – Link – Image @ Twitter Chat @ TECHNOLOGY https://science1984.wordpress.com/2023/01/08/internet-pioneers-17-equations-that-changed-the-world-history-of-the-world/ – Internet Pioneers – 17 Equations that Changed the World – History of the World – www.darpa.mil https://science1984.wordpress.com/2019/06/17/ja-que-rola-a-zueira-ha-muito-tempo-em-um-site-ok-tudo-bem-since-it-has-been-mess-a-long-time-at-a-website-ok-it-is-all-right-policiafederal-fbi-email-leitura-reading-internet-emails-wor/ https://science1984.wordpress.com/2019/06/17/ja-que-rola-a-zueira-ha-muito-tempo-em-um-site-ok-tudo-bem-since-it-has-been-mess-a-long-time-at-a-website-ok-it-is-all-right-policiafederal-fbi-email-leitura-reading-internet-emails-wor/ – Probability/Possibilidade (Email lists I did in 2015 and 2022) @ Email message and/or other situation: Who was the person who made the e-mail lists in the years of 2015 and/or 2022? @ As already mentioned by me on this Blog, few people didn’t like the fact that I put each person’s email address on the email list I created in 2022, informing me to unsubscribe. Therefore, there is a probability that someone will use an email list that I created and that someone who received an email message will also not like that their email was added to the email list (in this case, I created the email list in the year 2015 and 2022). Therefore, to avoid any type of problems for myself and other people, I will not send anyone any of the email lists I created, both the 2015 and the 2022 ones. As many know, the pioneering of the Internet took place in the USA, Therefore, this country has every right to decide everything regarding this issue, which is very important in several global aspects. It was a lot of hard work to create these email lists (2015 and 2022). I don’t want to be harmed in any way by this. The laws on this subject always have to be highly respected by people around the world for global progress. @ Conforme já mencionado por mim neste Blog, poucas pessoas não gostaram de eu ter colocado o email de cada uma na lista de emails que fiz em 2022, me informando para eu cancelar a inscrição. Portanto, há uma probabilidade de alguém usar alguma lista de emails que fiz e alguma pessoa que recebeu alguma mensagem de email também não gostar que colocaram o email dela na lista de emails (no caso, eu que fiz a lista de emails no ano de 2015 e 2022). Portanto, para evitar qualquer tipo de problemas para mim e também para outras pessoas, não irei enviar para algúem nenhuma das listas de emails que fiz, tanto a de 2015 como a de 2022. Como muitos sabem, o pioneirismo da Internet ocorreu nos EUA, portanto, este país tem todo o direito de decidir tudo do que se trata este assunto que é muito importante em vários aspectos mundiais. Foi um trabalho muito árduo fazer estas listas de emails (2015 e 2022). Não quero de nenhuma forma me prejudicar com isso. As leis sobre este assunto sempre tem que serem muito respeitadas pelas pessoas no mundo todo para o progresso mundial. & Feelings – Health – I/EU – Person/Pessoa – People/Pessoas – Thinking – Thoughts – Time/Tempo – Actions – Quality of Life – Times – Life – Countries – Human Lives – Human Relations – Internet @ Internet Society – Society & Different Types of Communication – World History – Scientific Discoveries https://science1984.wordpress.com/2023/01/08/probabilities-e-mail-content-no-money-email-message-and-or-other-situation-who-was-the-person-who-made-the-e-mail-lists-feelings-i-person-people-thinking-thoughts-time-actions/ – Internet – Gene – sem gene – cem genes – 100 genes – 100 – gene(s) – No Gene – No(s) Gene(s) – No Internet – 100 – Sem Internet – Vida(s) Humana(s) – Human Life – Grau(s) de Influência(s) – Level of Influence – No Communication – Sem Comunicação – Internet History – Test – Teste(s) – Tests – Internet Pioneer – UCLA/Stanford/Darpa – Internet Pioneers – Pioneiro(s) da Internet – País(es) – USA – Time/Tempo – Country – World – Countries – City – Cities – Person (Pessoa) – Other Person (Outra Pessoa) – Distance – Distância(s) – World – History – World History – History – Internet Society – Internet – Society – Internet Origin – Origem da Internet – Pioneiro(s) da Internet – Times – Time(s) – Tempo(s) – Human Lives – Human Memory – Memória(s) Humana(s) – Futuro – Future – Now – Agora – Passado – Past – Presente – Present – Idade(s) – Age – Ages – Human Longevity – Longevidade Humana @ President Biden Hosts a Bilateral Meeting with President Xi Jinping of China The White House – 2,06 mi de inscritos – 11/15/2023 (Today/Hoje) -> https://www.youtube.com/watch?v=XQnjwLKBX0M https://science1984.wordpress.com/2018/12/20/15362/ Hi Rodrigo,´´ ´´I visited your blog and found it very insightful. I appreciate the effort you put into sharing valuable content.´´ https://www.linkedin.com/in/nandan-kumar-duddukuri-8801133b/ Chief Scientific Officer at Innatura Scientific Pvt. Ltd Fala sobre #medicine, #drugdiscovery, #pharmaceuticals, ##leadoptimization e ##customchemicalsynthesisFala sobre hashtag medicine, hashtag drugdiscovery, hashtag pharmaceuticals, hashtag hashtag leadoptimization e hashtag hashtag customchemicalsynthesis Innatura Scientific Pvt. Ltd Hyderabad, Telanganá, Índia  Informações de contato 3.823 seguidores  + de 500 conexões Amr El-Refaey BS Pharm, BCBBS, MBA, CMI Level 7 Certificate, Amber YaelSylvia Balazs, e mais 53 conexões em comum Sobre ´´I am a chemist with over 10 years of experience in the biotech and pharmaceutical industries, including over 10 years of managing small molecule drug discovery projects with interdisciplinary teams. My experience includes progressing to the candidate drug stage.´´ ´´I am actively building new business-to-business(B2B) networks across Europe and North America through a large and expanding international network. I am promoting drug discovery services with a creative approach to new opportunities, including custom synthesis, medicinal chemistry, pre-clinical and clinical synthesis of new chemical entities (NCEs), and hit-to-lead optimization through fragment-based drug design.´´ ´´At Innatura Scientific, we specialize in custom chemical synthesis services and have extensive experience in the field of medicinal chemistry. Our expertise includes the synthesis of compounds at scales ranging from milligrams to multiple kilograms, as well as the production of drug intermediates, active pharmaceutical ingredients (API), and impurities.´´ ´´We are particularly interested in exploring business opportunities in the research and development of PROTACs (Proteolysis-Targeting Chimera) and promoting our custom chemical synthesis services worldwide. We are seeking to collaborate with biotech and pharmaceutical companies, as well as academic teams, to exchange knowledge and expertise in this field. Our goal is to work together to develop new PROTACs and support the discovery of new therapeutics.´´ ´´Additionally, we offer custom-targeted fluorescent probe services to help accelerate the discovery of new drugs.´´ ´´We believe that through collaboration, we can bring new and innovative solutions to the field of drug discovery. We look forward to the opportunity to work with leading companies and academic teams to achieve this goal.´´ ´´Our service models include fee-for-service (FFS), purchase orders (PO), full-time equivalent (FTE), and contract manufacturing. We are supported by a scientific advisory board from reputable institutions worldwide.´´ ´´Specialties: Project management, medicinal chemistry, synthetic chemistry, outsourced chemistry, pharmacology, telomerase inhibitor design, in vivo therapy experiments, pharmacokinetics, oncology, anti-aging, and structure-based drug design.´´ ´´Skills: Drug Development, Scientific Writing, Pharmacology, Patent Law, Validation, Good Manufacturing Practice (GMP), Good Laboratory Practice (GLP), Cancer, Life Sciences, Purification, Organic Chemistry, Laboratory Skills, Analytical Chemistry, Research and Development (R&D), Business Development.´´ Internet – World History/Time(s)/Data – Gene/Gene(s)/Genes – 100 – Sem Gene/No DNA/Sem DNA – Sem Genes – Cem Genes – 100 Genes – No Human Gene – Nos genes – No human genes – No(s) Gene(s) – No Human Cell – Sem Célula Humana – Sem Células Humanas/Sem Célula Humana – Cem Células Humanas – 100 human cells – 100 Células Humanas – No RNA/Sem RNA – No gene – No Internet/Sem Internet – Vida(s) Humana(s) – Human Life – Grau(s) de Influência(s) – Level of Influence – Level of Importance/Nível de Importância – No Communication – Sem Comunicação – Internet History – Test – Teste(s)/Teste – Tests – Internet Pioneers – UCLA/Stanford/Darpa – Pioneiros da Internet – Origin of the Internet – País(es) – USA – Time(s)/Tempo(s) – Country – World – Countries – City/Cities – Economy/Economia – Person/Pessoa – Other Person/Outra Pessoa – Distance/Distância(s) – Probabilidade de Leitura – Probability of Reading – Probabilities of Facts/Probabilidades de Fatos – World – History – World History – History – Internet Society – Internet – Society – Internet Origin – Origem da Internet – Time(s) – Tempo(s) – Human Lives – Human Memory – Memória(s) Humana(s) – Futuro – Future – Now – Agora – Passado – Past – Presente – Present – Idade(s) – Age – Ages – Human Longevity – Longevidade Humana @ President Biden Hosts a Bilateral Meeting with President Xi Jinping of China The White House – 2,06 mi de inscritos – 11/15/2023 (Today/Hoje) -> https://www.youtube.com/watch?v=XQnjwLKBX0M www.nih.gov www.cdc.gov www.fda.gov www.pfizer.com www.wordpress.com www.wikipedia.org www.google.com www.gmail.com www.youtube.com www.yahoo.com www.stripe.com www.forbes.com www.ft.com www.cnn.com www.bbc.com www.nyt.com www.wsj.com www.timeshighereducation.com www.nasa.gov www.genome.gov www.harvard.edu www.mit.edu www.caltech.edu www.columbia.edu www.yale.edu www.duke.edu www.famerp.br www.uftm.edu.br www.nasdaq.com www.fbi.gov www.cia.gov www.bankofamerica.com www.linkedin.com www.twitter.com www.instagram.com www.facebook.com www.spacex.com www.tesla.com www.ita.br www.embraer.com www.amazon.com www.princeton.edu www.whitehouse.gov www.usa.gov www.exame.com www.washingtonpost.com www.reuters.com www.abc.com www.wellsfargo.com www.worldbank.org www.nyu.edu www.cornell.edu www.nature.com www.nejm.com www.nobelprize.org https://science1984.wordpress.com/2018/12/20/15362/ ´´I am very happy that you shared this valuable information... it is really a valuable work that requires a lot of care... I sincerely congratulate you´´   ´´ ´´  Much success and health always for us !! For a better world always in all ways.https://www.linkedin.com/in/banu-sel%C3%A7uk-61213a24a/ ´´Hi Rodrigo,´´ ´´I visited your blog and found it very insightful. I appreciate the effort you put into sharing valuable content.´´https://www.linkedin.com/in/nandan-kumar-duddukuri-8801133b/ ´´Thank you very much, Rodrigo.´´´´ https://twitter.com/ClarineMichael8 @ClarineMichael8 Follows youHuman Rights Advocate, passionate about protection of religious freedoms & objective reporting. London, UKJoined October 2018 4,856 Following 2,291 Followers Followed by Matthew VanDyke, Alexander Verbeek  , and Svein Tveitdal´´Thanks for passing along´´ Much success and health always for us !! For a better world always in all ways.https://www.linkedin.com/in/cameroncoates1/details/experience/ ´´Wow, these are very helpful.´´´´Thanks,´´´´Hongshan´´´ Much success and health always for us !! For a better world always in all ways. https://www.linkedin.com/in/hsl2007/´´Thanks for sharing, Rodrigo´´  Much success and health always for us !! For a better world always in all ways.https://www.linkedin.com/in/fdatarobotkathy/ ´´ ´´ Much success and health always for us !! For a better world always in all ways.https://www.linkedin.com/in/sture-hobro-b858692/´´Thank you Rodrigo - will check out your blog  Thank you for sharing  ´´ Much success and health always for us !! For a better world always in all ways. ´´ ´´ https://www.linkedin.com/in/benpalmer369/  ´´Thank you very much´´Thank you so much for attention to me!Much success nd health always for us !! For a better always in all ways.´´This is exactly what I dedicate my time to… developing and bringing to the world technologies that contribute to the betterness of people.´´Many congratulations for excellent work Amnon !! As you know, the world people need to have very important ideas and initiatives always!!´´Thanks´´´´Can you convince the Municipal de São José do Rio Preto to utilize innovative solutions?´´It is a very good city in Brazil.* Much success and health always for us !! For a better world always in all ways.   https://www.linkedin.com/in/amnon-samid-3057418/ ´´Hi Rodrigo,´´´´Thanks for sharing. Was there something you were hoping I could help you with?´´´´Best,´´´´JDG´´If you could visit and share I will be very happy !!Thank you so much for attention to me! Much success and health always for us !! For a better world always in all ways.https://www.linkedin.com/in/jonathan-d-grinstein-phd-14424184/´´Thanks a lot´´ Thank you so much for attention to me! Much success nd health always for us !! For a better always in all ways.´´Thank you´´https://www.linkedin.com/in/claude-parent-6b000817/ ´´Dear Rodrigo´´ ´´Thank you for connecting – I look forward to following your thought leadership. Incidentally, I work a lot with Brazil. Our work, research and my teaching has a significant focus on Anticipatory Governance, Climate & Strategic Foresight, Sustainable Futures, and System Innovation. We also just launched our DFI Sustainability & Climate Academy. In case of interest, the Disruptive Futures Institute recently released our final volume of The Definitive Guide to Thriving on Disruption. This Volume IV “Disruption as a Springboard to Value Creation” jumped to bestseller and #1 new release in Green Business, Sustainable Economic Development, Business Education, Planning & Forecasting amongst others. You can stay connected with us in San Francisco. Feel free follow our Think Tank, the Disruptive Futures Institute on LinkedIn. We also have our dedicated YouTube Channel as well as Instagram & Twitter ( @disrupt_futures ). Kind regards, roger https://www.thrivingondisruption.com/the-books´´´´This is very impressive Rodrigo, thank you so much for sharing. These are indeed topics of interest to me. Look forward to discovering and sharing. Be well, roger´´Thank you so much Roger !! Maybe I can earn so much money with my blog doing a great partnership with someone in the world, for example, I can do a post about the possible partner that this post is the first to people see and read, when the person visit my blog that has much visibility and I like to share my blog post in my social networks like Facebook that I have almost the limit of friends (5000), LinkedIn, Twitter and Instagram. As you know, a partner need to have much visibility, and if the visibility is more especifically like the scientific community, better will be the partnership. I will send you some of very important links about this subject. If possible, share to your friends, maybe one of them has the interest in paying a great money for me monthly to I did a post about the partner and maybe the partnership will be forever. I never did a partnership with someone with my blog to I earn money monthly, for example. I never will delete my blog, of course. Very good connecting with you Roger!Much health, success and happiness for us always !! I hope get a very important partner with my blog for a long time or forever. If I do not get a partner, the most important thing is that I did the firs step, I tried, as you yknow. As I mentionated in my blog, the most important fact is that this blog is very valuable because so much people have visited and liked it, such as very important professors, researchers and/or scientists around the world. In relation to the use of a very important and extensive email list from people linked to Excellent Research and Teaching Centers Worldwide email list I did, so much people visit it every time and a very little amount of people informed me to unsubscribe since the first time I use it. As you know, the diffusion of relevant information and knowledge is always essential to the world progress in many aspects, for sure.Maybe a very important University in the USA can be a great partner with my blog and/or a very important industry and/or company about pharmacology, for example.Maybe a very important govern agency in the EUA can be interest in be a partner with my blog and/or other examples like communication companiesThanks for your attention Roger.´´Thanks Rodrigo for the additional elements. Very impressive, and appreciate the insights. I am not the right person/organization which can help, but wish you much success. Be well. roger´´Thank you so much Roger !!I did posts in a Facebook Group and in my Blog about your brilliant work informing for example, your Twitter and LinkedIn profiles, website and the positive feebacks I received from you for me about my Blog to people worldwide know about it, for sure ! Your work is very important to the world progress, of course!! Have a great day !!!´´Great Rodrigo, will have a look. Congrats!´´Thank You Roger !! https://www.linkedin.com/in/rogerspitz/ https://www.techistential.ai/rogerspitz https://www.linkedin.com/company/techistential/ https://twitter.com/techistential Thanks a lot´´ ´´Look like so helpful´´ Thank You !! Much success and health always for us !! For a better world always in all ways. Bilal Kalaycıoğlu   Conexão de 1º grau1º P.Eng Sirnak, Turquia  Informações de contato 314 conexões https://www.linkedin.com/in/bilal-kalayc%C4%B1o%C4%9Flu-39816a291/

34 Comments

  3. Once I originally commented I clicked the -Notify me when new comments are added- checkbox and now each time a comment is added I get four emails with the same comment. Is there any means you may take away me from that service? Thanks!

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  5. Very good blog! Do you have any hints for aspiring writers? I’m hoping to start my own blog soon but I’m a little lost on everything. Would you recommend starting with a free platform like WordPress or go for a paid option? There are so many options out there that I’m totally overwhelmed .. Any suggestions? Kudos!

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  19. Please let me know if you’re looking for a author for your blog. You have some really good articles and I believe I would be a good asset. If you ever want to take some of the load off, I’d love to write some content for your blog in exchange for a link back to mine. Please shoot me an email if interested. Many thanks!

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  20. Hello would you mind letting me know which web host you’re using? I’ve loaded your blog in 3 completely different internet browsers and I must say this blog loads a lot quicker then most. Can you recommend a good internet hosting provider at a honest price? Thanks, I appreciate it!

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  21. Heya! I just wanted to ask if you ever have any trouble with hackers? My last blog (wordpress) was hacked and I ended up losing months of hard work due to no back up. Do you have any solutions to prevent hackers?

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